Human Recombinant LSM1 protein, His Tag (V2LY-0526-LY5391)

Name: Human Recombinant LSM1 protein, His Tag
SKU: V2LY-0526-LY5391
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant LSM1 protein, His Tag consist of Amino Acid: 1-133 and predicts a molecular mass of 17 kDa.

Molecule Mass

17 kDa

Sequence

Amino Acid: 1-133

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

LSM1 homolog, U6 small nuclear RNA associated (S. cerevisiae)

Function

Plays a role in the degradation of histone mRNAs, the only eukaryotic mRNAs that are not polyadenylated (PubMed:18172165).
Probably also part of an LSm subunits-containing complex involved in the general process of mRNA degradation (By similarity).

Biological Process

Deadenylation-dependent decapping of nuclear-transcribed mRNAManual Assertion Based On ExperimentIBA:GO_Central
Histone mRNA catabolic processManual Assertion Based On ExperimentIMP:UniProtKB
mRNA processingManual Assertion Based On ExperimentTAS:ProtInc
Negative regulation of neuron differentiationIEA:Ensembl
RNA metabolic processManual Assertion Based On ExperimentIBA:GO_Central
RNA splicingManual Assertion Based On ExperimentTAS:ProtInc
RNA splicing, via transesterification reactionsManual Assertion Based On ExperimentTAS:UniProtKB
Stem cell population maintenanceIEA:Ensembl

Cellular Location

Cytoplasm
Cytoplasm, P-body

Montemayor, E. J., Virta, J. M., Hayes, S. M., Nomura, Y., Brow, D. A., & Butcher, S. E. (2020). Molecular basis for the distinct cellular functions of the Lsm1–7 and Lsm2–8 complexes. Rna, 26(10), 1400-1413.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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