Human Recombinant LYPLA2 protein, His Tag (V2LY-0526-LY5410)

Name: Human Recombinant LYPLA2 protein, His Tag
SKU: V2LY-0526-LY5410
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant LYPLA2 protein, His Tag consist of Amino Acid: 1-231 and predicts a molecular mass of 26.2 kDa.

Molecule Mass

26.2 kDa

Sequence

Amino Acid: 1-231

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>96% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

lysophospholipase II

Function

Acts as a acyl-protein thioesterase hydrolyzing fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins, GAP43, ZDHHC6 or HRAS (PubMed:21152083, PubMed:28826475).
Deacylates GAP43 (PubMed:21152083).
Mediates depalmitoylation of ZDHHC6 (PubMed:28826475).
Has lysophospholipase activity (PubMed:25301951).
Hydrolyzes prostaglandin glycerol esters (PG-Gs) in the following order prostaglandin D2-glycerol ester (PGD2-G)> prostaglandin E2 glycerol ester (PGE2-G)> prostaglandin F2-alpha-glycerol ester (PGF2-alpha-G) (PubMed:25301951).
Hydrolyzes 1-arachidonoylglycerol but not 2-arachidonoylglycerol or arachidonoylethanolamide (PubMed:25301951).

Biological Process

Acylglycerol catabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Axon guidanceTAS:Reactome
Fatty acid metabolic processIEA:UniProtKB-KW
Prostaglandin catabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Protein depalmitoylationManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Cytoplasm

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-LYPLA2 Recombinant Antibody (CBYJL-2253) (CAT#: CBMAB-L2513-YJ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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