Human Recombinant MAP2K3, Active protein, GST Tag (V2LY-0526-LY5526)

Name: Human Recombinant MAP2K3, Active protein, GST Tag
SKU: V2LY-0526-LY5526
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant MAP2K3, Active protein, GST Tag consist of Amino Acid: Full Length and predicts a molecular mass of 62 kDa.

Molecule Mass

62 kDa

Sequence

Amino Acid: Full Length

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

PBS

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

Mitogen-Activated Protein Kinase Kinase 3

Function

Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.

Biological Process

Cardiac muscle contractionIEA:Ensembl
Cellular response to lipopolysaccharideIEA:Ensembl
Cellular response to sorbitolIEA:Ensembl
Cellular response to vascular endothelial growth factor stimulusManual Assertion Based On ExperimentIMP:BHF-UCL
Cellular senescenceTAS:Reactome
Heart developmentIEA:Ensembl
Inflammatory responseIEA:Ensembl
MAPK cascadeManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of hippo signalingManual Assertion Based On ExperimentIMP:FlyBase
p38MAPK cascadeManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of blood vessel endothelial cell migrationManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of MAPK cascadeIEA:Ensembl
Positive regulation of nitric-oxide synthase biosynthetic processIEA:Ensembl
Positive regulation of protein kinase activityManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of cytokine productionIEA:Ensembl
Response to ischemiaIEA:Ensembl
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Cytosol
Membrane
Nucleoplasm

Involvement in disease

Defects in MAP2K3 may be involved in colon cancer.

PTM

Autophosphorylated. Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity (PubMed:8622669).
Phosphorylated by TAOK2 (PubMed:11279118).
(Microbial infection) Yersinia YopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.

Gu, K., Wang, T., Peng, L., & Zhao, Y. (2022). FIP-fve stimulates cell proliferation and enhances IL-2 release by activating MAP2K3/p38α (MAPK14) signaling pathway in Jurkat E6-1 cells. Frontiers in Nutrition, 9, 881924.

Bhargavi, M., Vhora, N., Lanka, G., Somadi, G., Kanth, S. S., Jain, A., & Potlapally, S. R. (2021). Homology modelling and virtual screening to explore potent inhibitors for MAP2K3 protein. Structural Chemistry, 32, 1039-1051.

Zhang, Y., Lu, W., Chen, Y., Lin, Y., Yang, X., Wang, H., & Liu, Z. (2021). The miR‐19b‐3p‐MAP2K3‐STAT3 feedback loop regulates cell proliferation and invasion in esophageal squamous cell carcinoma. Molecular Oncology, 15(5), 1566-1583.

Sawada, Y., Nakatsuji, T., Dokoshi, T., Kulkarni, N. N., Liggins, M. C., Sen, G., & Gallo, R. L. (2021). Cutaneous innate immune tolerance is mediated by epigenetic control of MAP2K3 by HDAC8/9. Science immunology, 6(59), eabe1935.

Qi, A., Wang, T., Li, W., Wang, Y., & Chai, Y. (2021). The effect of miR-21-5p on the MAP2K3 expressions and cellular apoptosis in the lung tissues of neonatal rats with hyperoxia-induced lung injuries. American Journal of Translational Research, 13(4), 2784.

Kim, Y. J., Jung, E., Shin, E., Hong, S. H., Jeong, H. S., Hur, G., ... & Kim, J. (2020). Genome-wide RNA interference screening reveals a COPI-MAP2K3 pathway required for YAP regulation. Proceedings of the National Academy of Sciences, 117(33), 19994-20003.

Gholipoorfeshkecheh, R., Agarwala, S., Krishnappa, S., Savitha, M. R., & Ramachandra, N. B. (2020). Whole-exome sequencing and homozygosity mapping identify variants in NCOR1 and MAP2K3 associated with non-syndromic congenital heart defects. Egyptian Journal of Medical Human Genetics, 21(1), 1-10.

Han, S., Wang, Y., Ma, J., Wang, Z., Wang, H. M. D., & Yuan, Q. (2020). Sulforaphene inhibits esophageal cancer progression via suppressing SCD and CDH3 expression, and activating the GADD45B-MAP2K3-p38-p53 feedback loop. Cell death & disease, 11(8), 713.

He, F., Xiao, Z., Yao, H., Li, S., Feng, M., Wang, W., ... & Wu, J. (2019). The protective role of microRNA-21 against coxsackievirus B3 infection through targeting the MAP2K3/P38 MAPK signaling pathway. Journal of translational medicine, 17(1), 1-11.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant MAP2K3 protein, GST Tag (CAT#: V2LY-0526-LY5525)

Human Recombinant MAP2K3 protein, His Tag (CAT#: V2LY-0526-LY5527)

Human Recombinant MAP2K3, Active protein, His Tag (CAT#: V2LY-0526-LY5528)

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Human Recombinant MAP2K3 protein, GST Tag (CAT#: V2LY-0526-LY5525)

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Isotype control

For research use only. Not intended for any clinical use.

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