Human Recombinant MAP3K2, Active protein, GST Tag (V2LY-0526-LY5436)

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Basic Information

Expressed Host
Baculovirus-Insect Cells
Protein Species
Human
Tag
GST Tag
Protein Construction
This product is Human Recombinant MAP3K2, Active protein, GST Tag consist of Amino Acid: Full Length and predicts a molecular mass of 115 kDa.
Molecule Mass
115 kDa
Sequence
Amino Acid: Full Length
Species
Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
Batch dependent.
Endotoxin
Please contact us for more information.
Format
Liquid
Buffer
MOPS, NaCl, Glutathione, DTT, PMSF, Glycerol
Preservative
None
Storage
Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
More Infomation

Target

Full Name
MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 2
Function
Component of a protein kinase signal transduction cascade. Regulates the JNK and ERK5 pathways by phosphorylating and activating MAP2K5 and MAP2K7 (By similarity).
Plays a role in caveolae kiss-and-run dynamics.
Biological Process
Cellular response to mechanical stimulusManual Assertion Based On ExperimentIEP:UniProtKB
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Protein phosphorylationManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Cytoplasm
Nucleus
Upon EGF stimulation, translocates into the nucleus.
PTM
Autophosphorylated.
Ubiquitination by XIAP/BIRC4 does not lead to proteasomal degradation.

Ikram, S., Rege, A., Negesse, M. Y., Casanova, A. G., Reynoird, N., & Green, E. M. (2023). The SMYD3-MAP3K2 signaling axis promotes tumor aggressiveness and metastasis in prostate cancer. Science Advances, 9(46), eadi5921.

Wan, Y., Huang, J., Song, Y., Gu, C., Kong, J., Zuo, L., & Chen, J. (2022). hsa-miR-340-5p inhibits epithelial–mesenchymal transition in endometriosis by targeting MAP3K2 and inactivating MAPK/ERK signaling. Open Medicine, 17(1), 566-576.

Liu, J., Xu, S., Liu, S., & Chen, B. (2021). miR‑3613‑3p/MAP3K2/p38/caspase‑3 pathway regulates the heat‑stress‑induced apoptosis of endothelial cells. Molecular Medicine Reports, 24(3), 1-13.

Yuan, Q., Basit, A., Liang, W., Qu, R., Luan, Y., Ren, C., ... & Wu, D. (2021). Pazopanib ameliorates acute lung injuries via inhibition of MAP3K2 and MAP3K3. Science translational medicine, 13(591), eabc2499.

Zhang, Y., Wang, D., Zhu, T., Yu, J., Wu, X., Lin, W., ... & Zhu, J. (2021). CircPUM1 promotes hepatocellular carcinoma progression through the miR‐1208/MAP3K2 axis. Journal of Cellular and Molecular Medicine, 25(1), 600-612.

Wu, N., Sun, H., Zhao, X., Zhang, Y., Tan, J., Qi, Y., ... & Su, B. (2021). MAP3K2-regulated intestinal stromal cells define a distinct stem cell niche. Nature, 592(7855), 606-610.

Liao, X., Wen, L., & Luo, L. (2021). The effect and mechanism of lncRNA NR2F1-As1/miR-493-5p/MAP3K2 axis in the progression of gastric cancer. Journal of Oncology, 2021, 1-10.

Shi, X., Liu, T. T., Yu, X. N., Balakrishnan, A., Zhu, H. R., Guo, H. Y., ... & Shen, X. Z. (2020). microRNA-93-5p promotes hepatocellular carcinoma progression via a microRNA-93-5p/MAP3K2/c-Jun positive feedback circuit. Oncogene, 39(35), 5768-5781.

Liu, L., Li, Y., Zhang, R., Li, C., Xiong, J., & Wei, Y. (2020). MIR205HG acts as a ceRNA to expedite cell proliferation and progression in lung squamous cell carcinoma via targeting miR-299-3p/MAP3K2 axis. BMC Pulmonary Medicine, 20(1), 1-9.

Wang, M., Lv, G., Jiang, C., Xie, S., & Wang, G. (2019). miR-302a inhibits human HepG2 and SMMC-7721 cells proliferation and promotes apoptosis by targeting MAP3K2 and PBX3. Scientific Reports, 9(1), 2032.

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For research use only. Not intended for any clinical use.

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