Human Recombinant MAX protein, GST Tag (V2LY-0526-LY5475)

Name: Human Recombinant MAX protein, GST Tag
SKU: V2LY-0526-LY5475
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant MAX protein, GST Tag consist of Amino Acid: 1-160 and predicts a molecular mass of 46.1 kDa.

Molecule Mass

46.1 kDa

Sequence

Amino Acid: 1-160

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

MAX

Function

Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC:MAX complex is a transcriptional activator, whereas the MAD:MAX complex is a repressor. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. Represses MYC transcriptional activity from E-box elements.

Biological Process

Cellular response to peptide hormone stimulus Source: Ensembl
Cellular response to starvation Source: Ensembl
Negative regulation of gene expression Source: Ensembl
Negative regulation of transcription by RNA polymerase II Source: NTNU_SB
Neuron apoptotic process Source: Ensembl
Positive regulation of transcription, DNA-templated Source: ComplexPortal
Positive regulation of transcription by RNA polymerase II Source: GO_Central
Protein-containing complex assembly Source: Ensembl
Regulation of transcription by RNA polymerase II Source: GO_Central
Response to axon injury Source: Ensembl
Response to insulin Source: Ensembl
Retina development in camera-type eye Source: Ensembl

Cellular Location

Nucleus
Other locations
dendrite

Involvement in disease

Pheochromocytoma (PCC):
A catecholamine-producing tumor of chromaffin tissue of the adrenal medulla or sympathetic paraganglia. The cardinal symptom, reflecting the increased secretion of epinephrine and norepinephrine, is hypertension, which may be persistent or intermittent.

PTM

Reversible lysine acetylation might regulate the nuclear-cytoplasmic shuttling of specific Max complexes.

Holmes, A. G., Parker, J. B., Sagar, V., Truica, M. I., Soni, P. N., Han, H., ... & Chakravarti, D. (2022). A MYC inhibitor selectively alters the MYC and MAX cistromes and modulates the epigenomic landscape to regulate target gene expression. Science Advances, 8(17), eabh3635.

Lafita-Navarro, M. C., Liaño-Pons, J., Quintanilla, A., Varela, I., Blanco, R., Ourique, F., ... & León, J. (2020). The MNT transcription factor autoregulates its expression and supports proliferation in MYC-associated factor X (MAX)-deficient cells. Journal of Biological Chemistry, 295(7), 2001-2017.

Li, K., Wang, F., Yang, Z. N., Zhang, T. T., Yuan, Y. F., Zhao, C. X., ... & Hu, Z. W. (2020). TRIB3 promotes MYC-associated lymphoma development through suppression of UBE3B-mediated MYC degradation. Nature Communications, 11(1), 6316.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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