Human Recombinant MFGE8 protein, His Tag (V2LY-0526-LY5574)

Name: Human Recombinant MFGE8 protein, His Tag
SKU: V2LY-0526-LY5574
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant MFGE8 protein, His Tag consist of Amino Acid: 1-387 and predicts a molecular mass of 42 kDa.

Molecule Mass

42 kDa

Sequence

Amino Acid: 1-387

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥80% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Sodium phosphate, NaCl, Imidazole, PMSF, DTT, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Milk Fat Globule-EGF Factor 8 Protein

Function

Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity).

Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction.

Medin:
Main constituent of aortic medial amyloid.

Biological Process

Angiogenesis Source: UniProtKB-KW
Apoptotic cell clearance Source: Ensembl
Cell adhesion Source: UniProtKB-KW
Phagocytosis, engulfment Source: Ensembl
Phagocytosis, recognition Source: Ensembl
Positive regulation of phagocytosis Source: Ensembl
Single fertilization Source: UniProtKB-KW

Cellular Location

Secreted
Other locations
Membrane
acrosome membrane
Note: Located in the acrosomal region of zona-pellucida bound sperm.

PTM

Medin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273.

Liu, B., Zhang, B., Qi, J., Zhou, H., Tan, L., Huang, J., ... & Guan, X. Y. (2023). Targeting MFGE8 secreted by cancer-associated fibroblasts blocks angiogenesis and metastasis in esophageal squamous cell carcinoma. Proceedings of the National Academy of Sciences, 120(42), e2307914120.

Ruotsalainen, S. E., Surakka, I., Mars, N., Karjalainen, J., Kurki, M., Kanai, M., ... & Southerington, T. (2022). Inframe insertion and splice site variants in MFGE8 associate with protection against coronary atherosclerosis. Communications biology, 5(1), 802.

Wang, J., Wang, Y., Zuo, Y., Duan, J., Pan, A., Li, J. M., ... & Liu, F. (2021). MFGE8 mitigates brain injury in a rat model of SAH by maintaining vascular endothelial integrity via TIGβ5/PI3K/CXCL12 signaling. Experimental Brain Research, 239(7), 2193-2205.

Tian, J., Wang, V., Wang, N., Khadang, B., Boudreault, J., Bakdounes, K., ... & Lebrun, J. J. (2021). Identification of MFGE8 and KLK5/7 as mediators of breast tumorigenesis and resistance to COX-2 inhibition. Breast Cancer Research, 23(1), 1-18.

Lim, H., Yang, T., Lee, W., & Park, S. G. (2021). TGF-β Increases MFGE8 production in myeloid-derived suppressor cells to promote B16F10 melanoma metastasis. Biomedicines, 9(8), 896.

Wang, B., Ge, Z., Wu, Y., Zha, Y., Zhang, X., Yan, Y., & Xie, Y. (2020). MFGE8 is down‐regulated in cardiac fibrosis and attenuates endothelial‐mesenchymal transition through Smad2/3‐Snail signalling pathway. Journal of cellular and molecular medicine, 24(21), 12799-12812.

Ni, Y. Q., Zhan, J. K., & Liu, Y. S. (2020). Roles and mechanisms of MFG-E8 in vascular aging-related diseases. Ageing Research Reviews, 64, 101176.

Ge, Z., Chen, Y., Wang, B., Zhang, X., Yan, Y., Zhou, L., ... & Xie, Y. (2020). MFGE8 attenuates Ang-II-induced atrial fibrosis and vulnerability to atrial fibrillation through inhibition of TGF-β1/Smad2/3 pathway. Journal of molecular and cellular cardiology, 139, 164-175.

Cheyuo, C., Aziz, M., & Wang, P. (2019). Neurogenesis in neurodegenerative diseases: role of MFG-E8. Frontiers in neuroscience, 13, 569.

Soubeyrand, S., Nikpay, M., Turner, A., Dang, A. T., Herfkens, M., Lau, P., & McPherson, R. (2019). Regulation of MFGE8 by the intergenic coronary artery disease locus on 15q26. 1. Atherosclerosis, 284, 11-17.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant MFGE8 protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY5573)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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