Human Recombinant NCF2 protein, GST Tag (V2LY-0526-LY5938)

Name: Human Recombinant NCF2 protein, GST Tag
SKU: V2LY-0526-LY5938
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant NCF2 protein, GST Tag consist of Amino Acid: 1-526 and predicts a molecular mass of 87.6 kDa.

Molecule Mass

87.6 kDa

Sequence

Amino Acid: 1-526

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Neutrophil Cytosolic Factor 2

Function

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

Biological Process

Cellular defense response Source: ProtInc
Innate immune response Source: BHF-UCL
Phagocytosis Source: InterPro
Respiratory burst Source: BHF-UCL
Superoxide anion generation Source: UniProtKB
Superoxide metabolic process Source: BHF-UCL

Cellular Location

Cytoplasm

Involvement in disease

Granulomatous disease, chronic, autosomal recessive, 2 (CGD2):
A form of chronic granulomatous disease, a primary immunodeficiency characterized by severe recurrent bacterial and fungal infections, along with manifestations of chronic granulomatous inflammation. It results from an impaired ability of phagocytes to mount a burst of reactive oxygen species in response to pathogens.

Huang, N., Zhang, J., Kuang, S., Li, Z., Zhao, H., Wu, J., ... & Wang, L. (2023). Role of NCF2 as a potential prognostic factor and immune infiltration indicator in hepatocellular carcinoma. Cancer Medicine, 12(7), 8991-9004.

Bakutenko, I. Y., Haurylchyk, I. D., Nikitchenko, N. V., Sechko, E. V., Kozyro, I. A., Tchitchko, A. M., ... & Ryabokon, N. I. (2022). Neutrophil cytosolic factor 2 (NCF2) gene polymorphism is associated with juvenile‐onset systemic lupus erythematosus, but probably not with other autoimmune rheumatic diseases in children. Molecular Genetics & Genomic Medicine, 10(1), e1859.

Azarova, I. E., Klyosova, E. Y., Kolomoets, I. I., & Polonikov, A. V. (2022). Polymorphic Variants of the Neutrophil Cytosolic Factor 2 Gene: Associations with Susceptibility to Type 2 Diabetes Mellitus and Cardiovascular Autonomic Neuropathy. Russian Journal of Genetics, 58(5), 593-602.

Roth, I. L., Salamon, P., Freund, T., Gadot, Y. B. D., Baron, S., Hershkovitz, T., ... & Hagin, D. (2020). Novel NCF2 mutation causing chronic granulomatous disease. Journal of Clinical Immunology, 40, 977-986.

Qin, K., Zheng, Z., He, Y., Gao, Y., Shi, H., Mo, S., ... & Rong, J. (2020). High expression of neutrophil cytosolic factor 2 (NCF2) is associated with aggressive features and poor prognosis of esophageal squamous cell carcinoma. International journal of clinical and experimental pathology, 13(12), 3033.

AlKhater, S. A., Deswarte, C., Casanova, J. L., & Bustamante, J. (2020). A novel variant in the neutrophil cytosolic factor 2 (NCF2) gene results in severe disseminated BCG infectious disease: A clinical report and literature review. Molecular genetics & genomic medicine, 8(6), e1237.

Wang, D., Liu, J., Chen, Q., Yang, R., & Jiang, Q. (2020). Upregulation of glutaminase 2 and neutrophil cytosolic factor 2 is associated with the poor prognosis of glioblastoma. Biomarkers in Medicine, 14(16), 1585-1597.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant NCF2 protein (CAT#: V2LY-0526-LY5937)

Human NCF2 ELISA Kit (CAT#: V2LY-0626-LY3998)

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Rabbit Anti-NCF2 Recombinant Antibody (CBWJN-1208) (CAT#: CBMAB-N1387-WJ)

Human Recombinant NCF2 protein (CAT#: V2LY-0526-LY5937)

Mouse Anti-NCF2 Recombinant Antibody (29/p67phox) (CAT#: CBMAB-P0539-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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