Human Recombinant NFASC protein, His Tag (V2LY-0526-LY5764)

Name: Human Recombinant NFASC protein, His Tag
SKU: V2LY-0526-LY5764
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant NFASC protein, His Tag consist of Amino Acid: 1-939 and predicts a molecular mass of 104.4 kDa.

Molecule Mass

104.4 kDa

Verified

HPLC

Sequence

Amino Acid: 1-939

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥85% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

neurofascin

Function

Cell adhesion, ankyrin-binding protein which may be involved in neurite extension, axonal guidance, synaptogenesis, myelination and neuron-glial cell interactions.

Biological Process

Axon guidance Source: GO_Central
Brain development Source: GO_Central
Cell-cell adhesion Source: GO_Central
Myelination Source: BHF-UCL
Peripheral nervous system development Source: BHF-UCL
Protein localization to plasma membrane Source: Ensembl
Synapse organization Source: InterPro
Transmission of nerve impulse Source: InterPro

Cellular Location

Plasma membrane
Cell membrane
Isoform 8:
Other locations
paranodal septate junction

Involvement in disease

Neurodevelopmental disorder with central and peripheral motor dysfunction (NEDCPMD):
An autosomal recessive neurodevelopmental disorder with early onset and a highly variable phenotype. Disease features include hypotonia apparent from birth, poor feeding, global developmental delay with absence of reaction to touch and no eye contact, infantile-onset progressive ataxia and demyelinating peripheral neuropathy.

Topology

Extracellular: 25-1217
Helical: 1218-1238
Cytoplasmic: 1239-1347

Chen, P., Yao, M., Fang, T., Ye, C., Du, Y., Jin, Y., & Wu, R. (2022). Identification of NFASC and CHL1 as two novel hub genes in endometriosis using integrated bioinformatic analysis and experimental verification. Pharmacogenomics and Personalized Medicine, 377-392.

Harper, J. L., Wilson, T. E., & Mitchell, R. M. (2020). Case report of two children with auditory neuropathy spectrum disorder related to a neurofascin (NFASC) gene variant. International Journal of Pediatric Otorhinolaryngology, 131, 109863.

Kvarnung, M., Shahsavani, M., Taylan, F., Moslem, M., Breeuwsma, N., Laan, L., ... & Falk, A. (2019). Ataxia in patients with bi-allelic NFASC mutations and absence of full-length NF186. Frontiers in genetics, 10, 896.

Wu, J., Huang, W. J., Xi, H. L., Liu, L. Y., Wang, S. T., Fan, W. Z., & Peng, B. G. (2019). Tumor-suppressive miR-3650 inhibits tumor metastasis by directly targeting NFASC in hepatocellular carcinoma. Aging (Albany NY), 11(11), 3432.

Monfrini, E., Straniero, L., Bonato, S., Compagnoni, G. M., Bordoni, A., Dilena, R., ... & Di Fonzo, A. (2019). Neurofascin (NFASC) gene mutation causes autosomal recessive ataxia with demyelinating neuropathy. Parkinsonism & Related Disorders, 63, 66-72.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant NFASC protein, hFc Tag (CAT#: V2LY-0526-LY5762)

Human Recombinant NFASC protein, Biotin Conjugated, hFc Tag (CAT#: V2LY-0526-LY5763)

Human NFASC ELISA Kit (CAT#: V2LY-0626-LY4947)

Related Products

Human Recombinant NFASC protein, Biotin Conjugated, hFc Tag (CAT#: V2LY-0526-LY5763)

Human Recombinant NFASC protein, hFc Tag (CAT#: V2LY-0526-LY5762)

Mouse Anti-NFASC Recombinant Antibody (CBWJN-1354) (CAT#: CBMAB-N2142-WJ)

Mouse Anti-NFASC Recombinant Antibody (CBWJN-1355) (CAT#: CBMAB-N2143-WJ)

Human NFASC ELISA Kit (CAT#: V2LY-0626-LY4947)

Rabbit Anti-NFASC Recombinant Antibody (D6G6O) (CAT#: CBMAB-CP1681-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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