Human Recombinant OLFM4 protein, His Tag (V2LY-0526-LY5869)

Name: Human Recombinant OLFM4 protein, His Tag
SKU: V2LY-0526-LY5869
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant OLFM4 protein, His Tag consist of Amino Acid: 1-510 and predicts a molecular mass of 56.6 kDa.

Molecule Mass

56.6 kDa

Sequence

Amino Acid: 1-510

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>92% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Olfactomedin 4

Function

May promote proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. In myeloid leukemic cell lines, inhibits cell growth and induces cell differentiation and apoptosis. May play a role in the inhibition of EIF4EBP1 phosphorylation/deactivation. Facilitates cell adhesion, most probably through interaction with cell surface lectins and cadherin.

Biological Process

Cell adhesionIEA:UniProtKB-KW
Positive regulation of substrate adhesion-dependent cell spreadingManual Assertion Based On ExperimentIDA:UniProtKB
Signal transductionManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Secreted, extracellular space
Mitochondrion
Subcellular location is not clearly defined: has been shown to be secreted (PubMed:16566923), but also in the mitochondrion (PubMed:15059901 and PubMed:20724538), cytoplasm and plasma membrane (PubMed:20724538) and in the nucleus (PubMed:15059901).

PTM

N-glycosylated.

Xu, K., Zheng, P., Zhao, S., Wang, J., Feng, J., Ren, Y., ... & Xie, P. (2023). LRFN5 and OLFM4 as novel potential biomarkers for major depressive disorder: a pilot study. Translational Psychiatry, 13(1), 188.

Liu, W., Li, H., Aerbajinai, W., Botos, I., & Rodgers, G. P. (2022). OLFM4-RET fusion is an oncogenic driver in small intestine adenocarcinoma. Oncogene, 41(1), 72-82.

Ren, X., Geng, M., Xu, K., Lu, C., Cheng, Y., Kong, L., ... & Xu, P. (2021). Quantitative proteomic analysis of synovial tissue reveals that upregulated OLFM4 aggravates inflammation in rheumatoid arthritis. Journal of proteome research, 20(10), 4746-4757.

Gong, F., Li, R., Zheng, X., Chen, W., Zheng, Y., Yang, Z., ... & Chen, E. (2021). OLFM4 regulates lung epithelial cell function in sepsis-associated ARDS/ALI via LDHA-mediated NF-κB signaling. Journal of inflammation research, 7035-7051.

Gao, X. Z., Wang, G. N., Zhao, W. G., Han, J., Diao, C. Y., Wang, X. H., ... & Li, W. C. (2019). Blocking OLFM4/HIF‐1α axis alleviates hypoxia‐induced invasion, epithelial–mesenchymal transition, and chemotherapy resistance in non‐small‐cell lung cancer. Journal of Cellular Physiology, 234(9), 15035-15043.

Valo, I., Raro, P., Boissard, A., Maarouf, A., Jézéquel, P., Verriele, V., ... & Guette, C. (2019). OLFM4 Expression in Ductal Carcinoma In Situ and in Invasive Breast Cancer Cohorts by a SWATH‐Based Proteomic Approach. Proteomics, 19(21-22), 1800446.

Fu, J., Dong, G., Shi, H., Zhang, J., Ning, Z., Bao, X., ... & Xiong, B. (2019). LncRNA MIR503HG inhibits cell migration and invasion via miR‐103/OLFM4 axis in triple negative breast cancer. Journal of cellular and molecular medicine, 23(7), 4738-4745.

Ashizawa, Y., Kuboki, S., Nojima, H., Yoshitomi, H., Furukawa, K., Takayashiki, T., ... & Ohtsuka, M. (2019). OLFM4 enhances STAT3 activation and promotes tumor progression by inhibiting GRIM19 expression in human hepatocellular carcinoma. Hepatology Communications, 3(7), 954-970.

Li, J., Liu, C., Li, D., Wan, M., Zhang, H., Zheng, X., ... & Sun, Q. (2019). OLFM4 inhibits epithelial–mesenchymal transition and metastatic potential of cervical cancer cells. Oncology Research, 27(7), 763.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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