Human Recombinant OTUB2 protein, His Tag (V2LY-0526-LY5895)

Name: Human Recombinant OTUB2 protein, His Tag
SKU: V2LY-0526-LY5895
Rating: 5 (1 reviews)

See all OTUB2 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant OTUB2 protein, His Tag consist of Amino Acid: 1-234 and predicts a molecular mass of 29 kDa.

Molecule Mass

29 kDa

Sequence

Amino Acid: 1-234

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>97% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

OTUB2

Function

Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of 'Lys-11'-,'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains.

Biological Process

Negative regulation of double-strand break repairManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of histone H2A K63-linked ubiquitinationManual Assertion Based On ExperimentIBA:GO_Central
Protein deubiquitinationManual Assertion Based On ExperimentIMP:UniProtKB
Protein K11-linked deubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Protein K48-linked deubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Protein K63-linked deubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Nucleus

Ren, W., Xu, Z., Chang, Y., Ju, F., Wu, H., Liang, Z., ... & Xia, N. (2024). Pharmaceutical targeting of OTUB2 sensitizes tumors to cytotoxic T cells via degradation of PD-L1. Nature Communications, 15(1), 9.

Xiao, J., Wang, L., Zhuang, Y., Zhu, Q., Li, W., Liao, H., ... & Liu, Z. (2024). The deubiquitinase OTUB2 promotes cervical cancer growth through stabilizing FOXM1. American Journal of Translational Research, 16(1), 75.

Zhang, Q., Zhang, J., Yao, A., Tian, X., Han, Z., Yuan, Y., ... & Yang, X. (2023). OTUB2 promotes the progression of endometrial cancer by regulating the PKM2‐mediated PI3K/AKT signaling pathway. Cell Biology International, 47(2), 428-438.

Ouyang, S., Zeng, Z., Liu, Z., Zhang, Z., Sun, J., Wang, X., ... & Kang, W. (2022). OTUB2 regulates KRT80 stability via deubiquitination and promotes tumour proliferation in gastric cancer. Cell Death Discovery, 8(1), 45.

Yu, S., Zang, W., Qiu, Y., Liao, L., & Zheng, X. (2022). Deubiquitinase OTUB2 exacerbates the progression of colorectal cancer by promoting PKM2 activity and glycolysis. Oncogene, 41(1), 46-56.

Hu, G., Yang, J., Zhang, H., Huang, Z., & Yang, H. (2022). OTUB2 promotes proliferation and migration of hepatocellular carcinoma cells by PJA1 deubiquitylation. Cellular and Molecular Bioengineering, 15(3), 281-292.

Sivakumar, D., Kumar, V., Naumann, M., & Stein, M. (2020). Activation and selectivity of OTUB-1 and OTUB-2 deubiquitinylases. Journal of Biological Chemistry, 295(20), 6972-6982.

Li, J., Cheng, D., Zhu, M., Yu, H., Pan, Z., Liu, L., ... & Yao, M. (2019). OTUB2 stabilizes U2AF2 to promote the Warburg effect and tumorigenesis via the AKT/mTOR signaling pathway in non-small cell lung cancer. Theranostics, 9(1), 179.

Zhang, Z., Du, J., Wang, S., Shao, L., Jin, K., Li, F., ... & Zhang, L. (2019). OTUB2 promotes cancer metastasis via hippo-independent activation of YAP and TAZ. Molecular cell, 73(1), 7-21.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human Recombinant OTUB2 protein, His Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human OTUB2 ELISA Kit (CAT#: V2LY-0626-LY5408)

Human Recombinant OTUB2, Active protein, His Tag (CAT#: V2LY-0526-LY5894)

Related Products

Mouse Anti-OTUB2 Recombinant Antibody (2F6) (CAT#: CBMAB-O0721-CQ)

Mouse Anti-OTUB2 Recombinant Antibody (CBT3143) (CAT#: V2LY-0625-LY1844)

Mouse Anti-OTUB2 Recombinant Antibody (CBXO-0208) (CAT#: CBMAB-O0722-CQ)

Mouse Anti-OTUB2 Recombinant Antibody (CBXO-0092) (CAT#: CBMAB-O0700-CQ)

Human OTUB2 ELISA Kit (CAT#: V2LY-0626-LY5408)

Mouse Anti-OTUB2 Recombinant Antibody (6F7) (CAT#: CBMAB-O0715-CQ)

Mouse Anti-OTUB2 Recombinant Antibody (CBXO-0207) (CAT#: CBMAB-O0716-CQ)

Rabbit Anti-OTUB2 Recombinant Antibody (CBXO-0465) (CAT#: CBMAB-O0723-CQ)

Mouse Anti-OTUB2 Antibody (9BA30) (CAT#: CBMAB-1477CQ)

Mouse Anti-OTUB2 (AA 126-235) Recombinant Antibody (2E7) (CAT#: CBMAB-O0714-CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry