Human OTUB2 ELISA Kit (V2LY-0626-LY5408)

See all OTUB2 antibodies
Go to compare Compare Online Inquiry
Tested Data
Datasheet
SDS
Request for COA
Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.0073 ng/mL
Detection Range
0.015-3 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
27.2 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
OTUB2
Function
Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of 'Lys-11'-,'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains.
Biological Process
Negative regulation of double-strand break repairManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of histone H2A K63-linked ubiquitinationManual Assertion Based On ExperimentIBA:GO_Central
Protein deubiquitinationManual Assertion Based On ExperimentIMP:UniProtKB
Protein K11-linked deubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Protein K48-linked deubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Protein K63-linked deubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Nucleus

Ren, W., Xu, Z., Chang, Y., Ju, F., Wu, H., Liang, Z., ... & Xia, N. (2024). Pharmaceutical targeting of OTUB2 sensitizes tumors to cytotoxic T cells via degradation of PD-L1. Nature Communications, 15(1), 9.

Xiao, J., Wang, L., Zhuang, Y., Zhu, Q., Li, W., Liao, H., ... & Liu, Z. (2024). The deubiquitinase OTUB2 promotes cervical cancer growth through stabilizing FOXM1. American Journal of Translational Research, 16(1), 75.

Zhang, Q., Zhang, J., Yao, A., Tian, X., Han, Z., Yuan, Y., ... & Yang, X. (2023). OTUB2 promotes the progression of endometrial cancer by regulating the PKM2‐mediated PI3K/AKT signaling pathway. Cell Biology International, 47(2), 428-438.

Ouyang, S., Zeng, Z., Liu, Z., Zhang, Z., Sun, J., Wang, X., ... & Kang, W. (2022). OTUB2 regulates KRT80 stability via deubiquitination and promotes tumour proliferation in gastric cancer. Cell Death Discovery, 8(1), 45.

Yu, S., Zang, W., Qiu, Y., Liao, L., & Zheng, X. (2022). Deubiquitinase OTUB2 exacerbates the progression of colorectal cancer by promoting PKM2 activity and glycolysis. Oncogene, 41(1), 46-56.

Hu, G., Yang, J., Zhang, H., Huang, Z., & Yang, H. (2022). OTUB2 promotes proliferation and migration of hepatocellular carcinoma cells by PJA1 deubiquitylation. Cellular and Molecular Bioengineering, 15(3), 281-292.

Sivakumar, D., Kumar, V., Naumann, M., & Stein, M. (2020). Activation and selectivity of OTUB-1 and OTUB-2 deubiquitinylases. Journal of Biological Chemistry, 295(20), 6972-6982.

Li, J., Cheng, D., Zhu, M., Yu, H., Pan, Z., Liu, L., ... & Yao, M. (2019). OTUB2 stabilizes U2AF2 to promote the Warburg effect and tumorigenesis via the AKT/mTOR signaling pathway in non-small cell lung cancer. Theranostics, 9(1), 179.

Zhang, Z., Du, J., Wang, S., Shao, L., Jin, K., Li, F., ... & Zhang, L. (2019). OTUB2 promotes cancer metastasis via hippo-independent activation of YAP and TAZ. Molecular cell, 73(1), 7-21.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Human OTUB2 ELISA Kit?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant OTUB2, Active protein, His Tag (CAT#: V2LY-0526-LY5894)

Human Recombinant OTUB2 protein, His Tag (CAT#: V2LY-0526-LY5895)

Related Products

Mouse Anti-OTUB2 Recombinant Antibody (CBT3143) (CAT#: V2LY-0625-LY1844)

Rabbit Anti-OTUB2 Recombinant Antibody (CBXO-0465) (CAT#: CBMAB-O0723-CQ)

Mouse Anti-OTUB2 Recombinant Antibody (6F7) (CAT#: CBMAB-O0715-CQ)

Mouse Anti-OTUB2 Recombinant Antibody (CBT3463) (CAT#: V2LY-0625-LY1843)

Mouse Anti-OTUB2 Recombinant Antibody (2E5) (CAT#: CBMAB-O0720-CQ)

Mouse Anti-OTUB2 Antibody (9BA30) (CAT#: CBMAB-1477CQ)

Human Recombinant OTUB2 protein, His Tag (CAT#: V2LY-0526-LY5895)

Human Recombinant OTUB2, Active protein, His Tag (CAT#: V2LY-0526-LY5894)

Mouse Anti-OTUB2 Recombinant Antibody (CBXO-0207) (CAT#: CBMAB-O0716-CQ)

Mouse Anti-OTUB2 Recombinant Antibody (CBXO-0208) (CAT#: CBMAB-O0722-CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry
Request bulk or custom quote Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

online inquiry
Online Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.