Human Recombinant PCBP1 protein, His Tag (V2LY-0526-LY5984)

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Basic Information

Expressed Host
E. coli
Protein Species
Human
Tag
His Tag
Protein Construction
This product is Human Recombinant PCBP1 protein, His Tag consist of Amino Acid: 2-356 and predicts a molecular mass of 38.3 kDa.
Molecule Mass
38.3 kDa
Sequence
Amino Acid: 2-356
Species
Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
>87% as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Format
Lyophilized
Reconstitution
Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.
Buffer
Lyophilized from sterile PBS
Preservative
None
Storage
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
More Infomation

Target

Full Name
Poly(RC) Binding Protein 1
Function
Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. In case of infection by poliovirus, plays a role in initiation of viral RNA replication in concert with the viral protein 3CD (PubMed:12414943).
Biological Process
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Regulation of gene expressionManual Assertion Based On ExperimentIBA:GO_Central
Regulation of RNA metabolic processManual Assertion Based On ExperimentIBA:GO_Central
Viral RNA genome replicationManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Nucleus
Cytoplasm
Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.
PTM
Phosphorylated; lowers poly(rC)-binding activity.

Wu, Y., Mou, J., Liu, Y., & Zheng, W. (2023). Association of LncRNA PCBP1-AS1 with cancer occurrence and development: A review. Medicine, 102(43), e35631.

Luo, Y., Zhang, Y., Pang, S., Min, J., Wang, T., Wu, D., ... & Ma, L. (2023). PCBP1 protects bladder cancer cells from mitochondria injury and ferroptosis by inducing LACTB mRNA degradation. Molecular Carcinogenesis, 62(7), 907-919.

Zhang, B., Zhang, M., Shen, C., Liu, G., Zhang, F., Hou, J., & Yao, W. (2021). LncRNA PCBP1-AS1-mediated AR/AR-V7 deubiquitination enhances prostate cancer enzalutamide resistance. Cell Death & Disease, 12(10), 856.

Jadhav, S., Protchenko, O., Li, F., Baratz, E., Shakoury-Elizeh, M., Maschek, A., ... & Philpott, C. C. (2021). Mitochondrial dysfunction in mouse livers depleted of iron chaperone PCBP1. Free Radical Biology and Medicine, 175, 18-27.

Huang, S., Luo, K., Jiang, L., Zhang, X. D., Lv, Y. H., & Li, R. F. (2021). PCBP1 regulates the transcription and alternative splicing of metastasis‑related genes and pathways in hepatocellular carcinoma. Scientific reports, 11(1), 23356.

Liao, C. Y., Lei, C. Q., & Shu, H. B. (2021). PCBP1 modulates the innate immune response by facilitating the binding of cGAS to DNA. Cellular & Molecular Immunology, 18(10), 2334-2343.

Ansa-Addo, E. A., Huang, H. C., Riesenberg, B., Iamsawat, S., Borucki, D., Nelson, M. H., ... & Li, Z. (2020). RNA binding protein PCBP1 is an intracellular immune checkpoint for shaping T cell responses in cancer immunity. Science advances, 6(22), eaaz3865.

Ishii, T., Igawa, T., Hayakawa, H., Fujita, T., Sekiguchi, M., & Nakabeppu, Y. (2020). PCBP1 and PCBP2 both bind heavily oxidized RNA but cause opposing outcomes, suppressing or increasing apoptosis under oxidative conditions. Journal of Biological Chemistry, 295(34), 12247-12261.

Luo, T., Gao, Y., Zhangyuan, G., Xu, X., Xue, C., Jin, L., ... & Qin, X. (2020). lncRNA PCBP1-AS1 aggravates the progression of hepatocellular carcinoma via regulating PCBP1/PRL-3/AKT pathway. Cancer Management and Research, 5395-5408.

Patel, S. J., Frey, A. G., Palenchar, D. J., Achar, S., Bullough, K. Z., Vashisht, A., ... & Philpott, C. C. (2019). A PCBP1–BolA2 chaperone complex delivers iron for cytosolic [2Fe–2S] cluster assembly. Nature chemical biology, 15(9), 872-881.

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For research use only. Not intended for any clinical use.

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