Human Recombinant PLEK protein, His Tag (V2LY-0526-LY6229)

Name: Human Recombinant PLEK protein, His Tag
SKU: V2LY-0526-LY6229
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant PLEK protein, His Tag consist of Amino Acid: 1-350 and predicts a molecular mass of 41.9 kDa.

Molecule Mass

41.9 kDa

Sequence

Amino Acid: 1-350

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

PLEK

Function

Major protein kinase C substrate of platelets.

Biological Process

Actin cytoskeleton reorganizationManual Assertion Based On ExperimentIDA:BHF-UCL
Cell projection organizationManual Assertion Based On ExperimentIDA:BHF-UCL
Cortical actin cytoskeleton organizationManual Assertion Based On ExperimentIDA:BHF-UCL
Hematopoietic progenitor cell differentiationManual Assertion Based On ExperimentIEP:BHF-UCL
Integrin-mediated signaling pathwayManual Assertion Based On ExperimentIDA:BHF-UCL
Negative regulation of calcium-mediated signaling1 PublicationNAS:BHF-UCL
Negative regulation of G protein-coupled receptor signaling pathwayManual Assertion Based On ExperimentIDA:BHF-UCL
Negative regulation of inositol phosphate biosynthetic processManual Assertion Based On ExperimentIDA:BHF-UCL
Phosphatidylinositol metabolic processManual Assertion Based On ExperimentIDA:BHF-UCL
Phospholipase C-inhibiting G protein-coupled receptor signaling pathwayManual Assertion Based On ExperimentIDA:BHF-UCL
Platelet aggregationManual Assertion Based On ExperimentHMP:UniProtKB
Platelet degranulationManual Assertion Based On ExperimentIEP:BHF-UCL
Positive regulation of actin filament bundle assemblyManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of actin filament depolymerizationManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of inositol-polyphosphate 5-phosphatase activityManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of integrin activationISS:BHF-UCL
Positive regulation of platelet activationISS:BHF-UCL
Protein kinase C signalingISS:BHF-UCL
Protein secretion by plateletISS:BHF-UCL
Regulation of cell diameterManual Assertion Based On ExperimentIDA:BHF-UCL
Ruffle organizationManual Assertion Based On ExperimentIDA:BHF-UCL
Thrombin-activated receptor signaling pathwayManual Assertion Based On ExperimentIDA:BHF-UCL
Vesicle docking involved in exocytosisISS:BHF-UCL

Cellular Location

cytoplasm
cytosol
extracellular region
membrane
plasma membrane
ruffle membrane

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

PLEK Matched Antibody Pair (887) (CAT#: APMAB-887LY)

PLEK Matched Antibody Pair (888) (CAT#: APMAB-888LY)

Antibody Pairs

PLEK Matched Antibody Pair (887) (CAT#: APMAB-887LY)

PLEK Matched Antibody Pair (888) (CAT#: APMAB-888LY)

Related Products

Mouse Anti-PLEK Recombinant Antibody (2D8) (CAT#: CBMAB-P2085-YC)

Mouse Anti-PLEK Recombinant Antibody (6E3) (CAT#: CBMAB-A6792-LY)

Mouse Anti-PLEK Recombinant Antibody (6B5) (CAT#: CBMAB-P2088-YC)

PLEK Matched Antibody Pair (887) (CAT#: APMAB-887LY)

Mouse Anti-PLEK Recombinant Antibody (3E2) (CAT#: CBMAB-P2087-YC)

PLEK Matched Antibody Pair (888) (CAT#: APMAB-888LY)

Mouse Anti-PLEK Recombinant Antibody (6E7) (CAT#: CBMAB-P2089-YC)

Mouse Anti-PLEK Recombinant Antibody (2F10) (CAT#: CBMAB-P2086-YC)

Mouse Anti-PLEK Recombinant Antibody (CBYC-P433) (CAT#: CBMAB-P2090-YC)

Mouse Anti-PLEK Recombinant Antibody (2D11) (CAT#: CBMAB-P2084-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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