Human Recombinant POSTN protein (V2LY-0526-LY6111)

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Datasheet
SDS
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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host
HEK293 Cells
Protein Species
Human
Protein Construction
This product is Human Recombinant POSTN protein consist of Amino Acid: 1-781 and predicts a molecular mass of 84.91 kDa.
Molecule Mass
84.91 kDa
Verified
HPLC
Sequence
Amino Acid: 1-781
Species
Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
≥95% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.
Endotoxin
Please contact us for more information.
Format
Lyophilized
Reconstitution
Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.
Buffer
Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol
Preservative
None
Storage
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
More Infomation

Target

Full Name
Periostin
Function
Induces cell attachment and spreading and plays a role in cell adhesion (PubMed:12235007).
Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity).
Biological Process
Cell adhesionManual Assertion Based On ExperimentIDA:UniProtKB
Cellular response to vitamin KManual Assertion Based On ExperimentIDA:UniProtKB
Extracellular matrix organizationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of Notch signaling pathwayIEA:Ensembl
Tissue developmentIEA:Ensembl
Cellular Location
Golgi apparatus
Secreted
Secreted, extracellular space, extracellular matrix
Colocalizes with BMP1 in the Golgi.
PTM
Gamma-carboxylation is controversial. Gamma-carboxyglutamated; gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation; this may be required for calcium binding (PubMed:18450759).
According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues (PubMed:26273833).
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Postn ELISA Kit (CAT#: V2LY-0626-LY2854)

Human POSTN ELISA Kit (CAT#: V2LY-0626-LY3702)

Human Recombinant POSTN protein, His Tag (CAT#: V2LY-0526-LY6110)

Mouse Recombinant POSTN protein, His Tag (CAT#: V2LY-0526-LY8767)

Related Products

Mouse Anti-POSTN (AA 1-230) Recombinant Antibody (CBXO-0536) (CAT#: CBMAB-O0643-CQ)

Human POSTN ELISA Kit (CAT#: V2LY-0626-LY3702)

Mouse Recombinant POSTN protein, His Tag (CAT#: V2LY-0526-LY8767)

Rat Anti-POSTN Recombinant Antibody (CBXO-0280) (CAT#: CBMAB-O0645-CQ)

Human Recombinant POSTN protein, His Tag (CAT#: V2LY-0526-LY6110)

Mouse Anti-POSTN Recombinant Antibody (F-10) (CAT#: CBMAB-P2421-YC)

Rat Anti-POSTN Recombinant Antibody (8H152) (CAT#: CBMAB-P2419-YC)

Mouse Anti-POSTN Recombinant Antibody (2D10) (CAT#: CBMAB-P2417-YC)

Mouse Anti-POSTN Recombinant Antibody (47) (CAT#: CBMAB-P2418-YC)

Mouse Anti-POSTN Recombinant Antibody (1A1) (CAT#: CBMAB-P2415-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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