Human Recombinant PRAP1 protein, Fc Tag (V2LY-0526-LY6289)

Name: Human Recombinant PRAP1 protein, Fc Tag
SKU: V2LY-0526-LY6289
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

Fc Tag

Protein Construction

This product is Human Recombinant PRAP1 protein, Fc Tag consist of Amino Acid: 1-151 and predicts a molecular mass of 41.4 kDa.

Molecule Mass

41.4 kDa

Sequence

Amino Acid: 1-151

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

proline-rich acidic protein 1

Function

Lipid-binding protein which promotes lipid absorption by facilitating MTTP-mediated lipid transfer (mainly triglycerides and phospholipids) and MTTP-mediated apoB lipoprotein assembly and secretion (By similarity).
Protects the gastrointestinal epithelium from irradiation-induced apoptosis (By similarity).
May play an important role in maintaining normal growth homeostasis in epithelial cells (PubMed:14583459).
Involved in p53/TP53-dependent cell survival after DNA damage (PubMed:23235459).
May down-regulate the expression of MAD1L1 and exert a suppressive role in mitotic spindle assembly checkpoint in hepatocellular carcinomas (PubMed:24374861).

Biological Process

Cellular response to DNA damage stimulusManual Assertion Based On ExperimentIDA:UniProtKB
Cellular response to X-rayISS:UniProtKB
Deactivation of mitotic spindle assembly checkpointManual Assertion Based On ExperimentIDA:UniProtKB
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrestManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of apoptotic processManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of intestinal lipid absorptionISS:UniProtKB
Positive regulation of phospholipid transportISS:UniProtKB
Positive regulation of triglyceride transportISS:UniProtKB

Cellular Location

Secreted
Endoplasmic reticulum

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant PRAP1 protein, His Tag (CAT#: V2LY-0526-LY6288)

Related Products

Human Recombinant PRAP1 protein, His Tag (CAT#: V2LY-0526-LY6288)

Mouse Anti-PRAP1 Recombinant Antibody (2B4) (CAT#: CBMAB-A6993-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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