Human Recombinant RAB31 protein, His Tag (V2LY-0526-LY6436)

Name: Human Recombinant RAB31 protein, His Tag
SKU: V2LY-0526-LY6436
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant RAB31 protein, His Tag consist of Amino Acid: 1-195 and predicts a molecular mass of 23.9 kDa.

Molecule Mass

23.9 kDa

Sequence

Amino Acid: 1-195

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

RAB31, Member RAS Oncogene Family

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Required for the integrity and for normal function of the Golgi apparatus and the trans-Golgi network. Plays a role in insulin-stimulated translocation of GLUT4 to the cell membrane. Plays a role in M6PR transport from the trans-Golgi network to endosomes. Plays a role in the internalization of EGFR from the cell membrane into endosomes. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis.

Biological Process

Biological Process cellular response to insulin stimulusManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process Golgi to plasma membrane protein transportManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process intracellular protein transportManual Assertion Based On ExperimentIBA:GO_Central
Biological Process phagosome maturationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process positive regulation of phagocytosis, engulfmentISS:UniProtKB
Biological Process receptor internalizationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulated exocytosisManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Golgi apparatus, trans-Golgi network
Golgi apparatus, trans-Golgi network membrane
Early endosome
Cytoplasmic vesicle, phagosome
Cytoplasmic vesicle, phagosome membrane
Rapidly recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

RAB31 Matched Antibody Pair (956) (CAT#: APMAB-956LY)

Antibody Pairs

RAB31 Matched Antibody Pair (956) (CAT#: APMAB-956LY)

Related Products

RAB31 Matched Antibody Pair (956) (CAT#: APMAB-956LY)

Mouse Anti-RAB31 Recombinant Antibody (4D12) (CAT#: CBMAB-R0816-CN)

Mouse Anti-RAB31 Recombinant Antibody (CBCNR-032) (CAT#: CBMAB-R0817-CN)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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