Human Recombinant RCC1 protein, His Tag (V2LY-0526-LY6477)

Name: Human Recombinant RCC1 protein, His Tag
SKU: V2LY-0526-LY6477
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant RCC1 protein, His Tag consist of Amino Acid: Full Length and predicts a molecular mass of 50 kDa.

Molecule Mass

50 kDa

Sequence

Amino Acid: Full Length

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

PBS

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

Regulator Of Chromosome Condensation 1

Function

Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP, and thereby plays an important role in RAN-mediated functions in nuclear import and mitosis (PubMed:1944575, PubMed:17435751, PubMed:20668449, PubMed:22215983, PubMed:11336674).
Contributes to the generation of high levels of chromosome-associated, GTP-bound RAN, which is important for mitotic spindle assembly and normal progress through mitosis (PubMed:12194828, PubMed:17435751, PubMed:22215983).
Via its role in maintaining high levels of GTP-bound RAN in the nucleus, contributes to the release of cargo proteins from importins after nuclear import (PubMed:22215983).
Involved in the regulation of onset of chromosome condensation in the S phase (PubMed:3678831).
Binds both to the nucleosomes and double-stranded DNA (PubMed:17435751, PubMed:18762580).

Biological Process

Cell divisionIEA:UniProtKB-KW
Chromosome segregationManual Assertion Based On ExperimentIMP:UniProtKB
G1/S transition of mitotic cell cycleManual Assertion Based On ExperimentIMP:UniProtKB
Mitotic nuclear membrane reassemblyTAS:Reactome
Mitotic spindle organizationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of mitotic nuclear divisionManual Assertion Based On ExperimentIDA:UniProtKB
Spindle assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Viral processTAS:Reactome

Cellular Location

Nucleus
Chromosome
Cytoplasm
Predominantly nuclear in interphase cells (PubMed:12194828).
Binds to mitotic chromosomes (PubMed:12194828, PubMed:17435751, PubMed:20668449).

PTM

N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di- and trimethylation produce a permanent positive charge on the amino group, which facilitates electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

RCC1 Matched Antibody Pair (976) (CAT#: APMAB-976LY)

Antibody Pairs

RCC1 Matched Antibody Pair (976) (CAT#: APMAB-976LY)

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RCC1 Matched Antibody Pair (976) (CAT#: APMAB-976LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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