Human Recombinant SEMA5A protein, His Tag (V2LY-0526-LY6708)

Name: Human Recombinant SEMA5A protein, His Tag
SKU: V2LY-0526-LY6708
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant SEMA5A protein, His Tag consist of Amino Acid: 1-968 and predicts a molecular mass of 107 kDa.

Molecule Mass

107 kDa

Sequence

Amino Acid: 1-968

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Semaphorin 5A

Function

Bifunctional axonal guidance cue regulated by sulfated proteoglycans; attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs) (By similarity).
Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis.

Biological Process

Biological Process axon extensionManual Assertion Based On ExperimentIBA:GO_Central
Biological Process axon guidanceManual Assertion Based On ExperimentIBA:GO_Central
Biological Process axonal fasciculationISS:UniProtKB
Biological Process blood vessel endothelial cell proliferation involved in sprouting angiogenesisISS:UniProtKB
Biological Process cell adhesionManual Assertion Based On ExperimentTAS:ProtInc
Biological Process cell chemotaxisISS:UniProtKB
Biological Process cell-cell signalingManual Assertion Based On ExperimentTAS:ProtInc
Biological Process diencephalon developmentISS:UniProtKB
Biological Process negative regulation of axon extension involved in axon guidanceISS:UniProtKB
Biological Process negative regulation of cell adhesionISS:UniProtKB
Biological Process negative regulation of endothelial cell apoptotic processISS:UniProtKB
Biological Process nervous system developmentManual Assertion Based On ExperimentTAS:ProtInc
Biological Process neural crest cell migrationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process positive chemotaxisISS:UniProtKB
Biological Process positive regulation of actin filament depolymerizationISS:UniProtKB
Biological Process positive regulation of angiogenesisISS:UniProtKB
Biological Process positive regulation of axon extension involved in axon guidanceISS:UniProtKB
Biological Process positive regulation of canonical Wnt signaling pathwayISS:UniProtKB
Biological Process positive regulation of cell migrationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process positive regulation of endothelial cell chemotaxisISS:UniProtKB
Biological Process positive regulation of endothelial cell proliferationISS:UniProtKB
Biological Process positive regulation of protein kinase B signalingISS:UniProtKB
Biological Process semaphorin-plexin signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Biological Process signal clusteringISS:UniProtKB

Cellular Location

Membrane

Topology

Extracellular: 23-968
Helical: 969-989
Cytoplasmic: 990-1074

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant SEMA5A protein, hFc Tag (CAT#: V2LY-0526-LY6707)

Mouse Recombinant SEMA5A protein, His Tag (CAT#: V2LY-0526-LY8877)

Human SEMA5A ELISA Kit (CAT#: V2LY-0626-LY2184)

Related Products

Human Recombinant SEMA5A protein, hFc Tag (CAT#: V2LY-0526-LY6707)

Human SEMA5A ELISA Kit (CAT#: V2LY-0626-LY2184)

Mouse Anti-SEMA5A Recombinant Antibody (CBXS-1977) (CAT#: CBMAB-S4748-CQ)

Mouse Recombinant SEMA5A protein, His Tag (CAT#: V2LY-0526-LY8877)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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