Human Recombinant SIRPA (G75A) protein, ECD, His Tag (V2LY-0526-LY6823)

Name: Human Recombinant SIRPA (G75A) protein, ECD, His Tag
SKU: V2LY-0526-LY6823
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant SIRPA (G75A) protein, ECD, His Tag consist of Amino Acid: 1-370, 75 and predicts a molecular mass of 39 kDa.

Molecule Mass

39 kDa

Protein Domain

ECD

Verified

HPLC

Sequence

Amino Acid: 1-370, 75

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>98% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

signal regulatory protein alpha

Alternative Names

Signal Regulatory Protein Alpha; CD172 Antigen-Like Family Member A; Inhibitory Receptor SHPS-1; Macrophage Fusion Receptor; PTPNS1; SHPS1; SIRP; P84; BIT; MFR; Brain-Immunoglobulin-Like Molecule With Tyrosine-Based Activation Motifs; Brain Ig-Like Molecule With Tyrosine-Based Activation Motifs; Protein Tyrosine Phosphatase; Non-Receptor Type Substrate 1; Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1; Tyrosine Phosphatase SHP Substrate 1; Signal-Regulatory Protein Alpha-1;

Function

Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity).
Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.

Biological Process

Biological Process cell adhesionManual Assertion Based On ExperimentTAS:ProtInc
Biological Process cell migrationISS:ARUK-UCL
Biological Process cellular response to hydrogen peroxideISS:ARUK-UCL
Biological Process cellular response to interferon-gammaISS:ARUK-UCL
Biological Process cellular response to interleukin-1ISS:ARUK-UCL
Biological Process cellular response to interleukin-12Manual Assertion Based On ExperimentIMP:ARUK-UCL
Biological Process cellular response to lipopolysaccharideISS:ARUK-UCL
Biological Process monocyte extravasationISS:ARUK-UCL
Biological Process negative regulation of chemokine (C-C motif) ligand 5 productionISS:ARUK-UCL
Biological Process negative regulation of cytokine production involved in inflammatory responseISS:ARUK-UCL
Biological Process negative regulation of ERK1 and ERK2 cascadeISS:ARUK-UCL
Biological Process negative regulation of I-kappaB phosphorylationISS:ARUK-UCL
Biological Process negative regulation of inflammatory responseManual Assertion Based On ExperimentIMP:ARUK-UCL
Biological Process negative regulation of interferon-beta productionISS:ARUK-UCL
Biological Process negative regulation of interleukin-6 productionISS:ARUK-UCL
Biological Process negative regulation of JNK cascadeISS:ARUK-UCL
Biological Process negative regulation of macrophage inflammatory protein 1 alpha productionISS:ARUK-UCL
Biological Process negative regulation of nitric oxide biosynthetic processISS:ARUK-UCL
Biological Process negative regulation of phagocytosisISS:ARUK-UCL
Biological Process negative regulation of protein phosphorylationISS:ARUK-UCL
Biological Process negative regulation of tumor necrosis factor productionISS:ARUK-UCL
Biological Process positive regulation of phagocytosisManual Assertion Based On ExperimentIBA:GO_Central
Biological Process positive regulation of T cell activationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process regulation of gene expressionManual Assertion Based On ExperimentIMP:ARUK-UCL
Biological Process regulation of interferon-gamma productionManual Assertion Based On ExperimentIMP:ARUK-UCL
Biological Process regulation of interleukin-1 beta productionISS:ARUK-UCL
Biological Process regulation of interleukin-6 productionISS:ARUK-UCL
Biological Process regulation of nitric oxide biosynthetic processISS:ARUK-UCL
Biological Process regulation of tumor necrosis factor productionManual Assertion Based On ExperimentIGI:ARUK-UCL

Cellular Location

Membrane

Topology

Extracellular: 31-373
Helical: 374-394
Cytoplasmic: 395-504

PTM

N-glycosylated.
Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Cynomolgus Recombinant SIRPA protein, His Tag (CAT#: V2LY-0526-LY1640)

Human Recombinant SIRPA protein, ECD, hFc Tag (CAT#: V2LY-0526-LY6815)

Human Recombinant SIRPA protein, ECD, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY6816)

Human Recombinant SIRPA protein, ECD, His Tag (CAT#: V2LY-0526-LY6817)

Human Recombinant SIRPA (SIRP alphaV2) protein, ECD, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY6818)

Human Recombinant SIRPA (SIRP alphaV2) protein, ECD, His Tag (CAT#: V2LY-0526-LY6819)

Human Recombinant SIRPA (SIRP alphaV2) protein, ECD, Fc Tag (CAT#: V2LY-0526-LY6820)

Human Recombinant SIRPA (SIRP alphaV8) protein, ECD, His Tag (CAT#: V2LY-0526-LY6821)

Human Recombinant SIRPA (SIRP alphaV8) protein, ECD, Fc Tag (CAT#: V2LY-0526-LY6822)

Human Recombinant SIRPA protein, Biotin Conjugated, hFc & AVI Tag (CAT#: V2LY-0526-LY6824)

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Isotype control

For research use only. Not intended for any clinical use.

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