Human Recombinant SMYD3 protein, His & Flag Tag (V2LY-0526-LY6899)

Name: Human Recombinant SMYD3 protein, His & Flag Tag
SKU: V2LY-0526-LY6899
Rating: 5 (1 reviews)

See all SMYD3 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

His & Flag Tag

Protein Construction

This product is Human Recombinant SMYD3 protein, His & Flag Tag consist of Amino Acid: 1-428 and predicts a molecular mass of 51.67 kDa.

Molecule Mass

51.67 kDa

Sequence

Amino Acid: 1-428

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

SET And MYND Domain Containing 3

Function

Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation (PubMed:15235609, PubMed:22419068).
Also methylates 'Lys-5' of histone H4 (PubMed:22419068).
Plays an important role in transcriptional activation as a member of an RNA polymerase complex (PubMed:15235609).
Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences (PubMed:15235609).

Biological Process

Biological Process cellular response to dexamethasone stimulusIEA:Ensembl
Biological Process establishment of protein localizationIEA:Ensembl
Biological Process histone lysine methylationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process myotube cell developmentIEA:Ensembl
Biological Process negative regulation of protein kinase activityIEA:Ensembl
Biological Process nucleosome assemblyIEA:Ensembl
Biological Process positive regulation of peptidyl-serine phosphorylationIEA:Ensembl
Biological Process positive regulation of transcription by RNA polymerase IIIEA:Ensembl

Cellular Location

Cytoplasm
Nucleus
Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human Recombinant SMYD3 protein, His & Flag Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant SMYD3, Active protein, GST Tag (CAT#: V2LY-0526-LY6897)

Human Recombinant SMYD3 protein, GST Tag (CAT#: V2LY-0526-LY6898)

Related Products

Mouse Anti-SMYD3 Recombinant Antibody (CBXS-0587) (CAT#: CBMAB-S3529-CQ)

Rabbit Anti-SMYD3 Recombinant Antibody (D2Q4V) (CAT#: CBMAB-CP2523-LY)

Mouse Anti-SMYD3 Recombinant Antibody (CBXS-0586) (CAT#: CBMAB-S3528-CQ)

Mouse Anti-SMYD3 Recombinant Antibody (CBXS-2095) (CAT#: CBMAB-S4860-CQ)

Rabbit Anti-SMYD3 Recombinant Antibody (CBXS-1271) (CAT#: CBMAB-S4130-CQ)

Human Recombinant SMYD3 protein, GST Tag (CAT#: V2LY-0526-LY6898)

Human Recombinant SMYD3, Active protein, GST Tag (CAT#: V2LY-0526-LY6897)

Mouse Anti-SMYD3 Recombinant Antibody (CBXS-3600) (CAT#: CBMAB-S0886-CQ)

Rabbit Anti-SMYD3 Recombinant Antibody (CBXS-1454) (CAT#: CBMAB-S4311-CQ)

Rabbit Anti-SMYD3 Recombinant Antibody (CBXS-5373) (CAT#: CBMAB-S2586-CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry