Human Recombinant ST8SIA1 protein, His Tag (V2LY-0526-LY6961)

Name: Human Recombinant ST8SIA1 protein, His Tag
SKU: V2LY-0526-LY6961
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant ST8SIA1 protein, His Tag consist of Amino Acid: 49-356 and predicts a molecular mass of 36.6 kDa.

Molecule Mass

36.6 kDa

Verified

HPLC

Sequence

Amino Acid: 49-356

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS, Glycerol, Trehalose

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

ST8 Alpha-N-Acetyl-Neuraminide Alpha-2,8-Sialyltransferase 1

Function

Catalyzes the addition of sialic acid in alpha 2,8-linkage to the sialic acid moiety of the ganglioside GM3 to form ganglioside GD3; gangliosides are a subfamily of complex glycosphinglolipds that contain one or more residues of sialic acid (PubMed:7937974, PubMed:8058740, PubMed:8195250, PubMed:8631981, PubMed:8706663, PubMed:18348864, PubMed:22885356).
Can catalyze the addition of a second alpha-2,8-sialic acid to GD3 to form GT3 (PubMed:8631981).
Can use GM1b, GD1a and GT1b as acceptor substrates to synthesize GD1c, GT1a and GQ1b respectively (PubMed:8706663).
Can synthesize unusual tetra- and pentasialylated lactosylceramide derivatives identified as GQ3 (II3Neu5Ac4-Gg2Cer) and GP3 (II3Neu5Ac5-Gg2Cer) in breast cancer cells (PubMed:22885356).

Biological Process

Biological Process carbohydrate metabolic processManual Assertion Based On ExperimentTAS:ProtInc
Biological Process cellular response to heatIEA:Ensembl
Biological Process epithelial cell proliferationIEA:Ensembl
Biological Process glycosphingolipid biosynthetic processManual Assertion Based On ExperimentTAS:ProtInc
Biological Process N-glycan processingManual Assertion Based On ExperimentIBA:GO_Central
Biological Process oligosaccharide metabolic processManual Assertion Based On ExperimentIBA:GO_Central
Biological Process positive regulation of epithelial cell proliferationIEA:Ensembl
Biological Process protein glycosylationManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Golgi apparatus membrane

Topology

Cytoplasmic: 1-29
Helical: 30-48
Lumenal: 49-356

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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