Human Recombinant TLE3 protein, GST Tag (V2LY-0526-LY7261)

Name: Human Recombinant TLE3 protein, GST Tag
SKU: V2LY-0526-LY7261
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant TLE3 protein, GST Tag consist of Amino Acid: 484-772 and predicts a molecular mass of 58.5 kDa.

Molecule Mass

58.5 kDa

Sequence

Amino Acid: 484-772

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>78% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

TLE3 Transducin Like Enhancer Of Split 3

Function

Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (By similarity).

Biological Process

Biological Process animal organ morphogenesisSource:ProtInc1 Publication
Biological Process negative regulation of canonical Wnt signaling pathwaySource:GO_Central1 Publication
Biological Process negative regulation of cold-induced thermogenesisSource:YuBioLabBy Similarity
Biological Process signal transductionSource:ProtInc1 Publication
Biological Process Wnt signaling pathwaySource:UniProtKB-KW

Cellular Location

Nucleus

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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