Human Recombinant UBE2D1 protein, His Tag (V2LY-0526-LY7485)

Name: Human Recombinant UBE2D1 protein, His Tag
SKU: V2LY-0526-LY7485
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant UBE2D1 protein, His Tag consist of Amino Acid: 2-147 and predicts a molecular mass of 18.3 kDa.

Molecule Mass

18.3 kDa

Sequence

Amino Acid: 2-147

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

UBE2D1

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:22496338).
In vitro catalyzes 'Lys-48'-linked polyubiquitination (PubMed:20061386).
Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1 (PubMed:18042044, PubMed:18359941).
Ubiquitinates STUB1-associated HSP90AB1 in vitro (PubMed:18042044).
Lacks inherent specificity for any particular lysine residue of ubiquitin (PubMed:18042044).
Essential for viral activation of IRF3 (PubMed:19854139).
Mediates polyubiquitination of CYP3A4 (PubMed:19103148).

Biological Process

Biological Process negative regulation of BMP signaling pathway Source:Reactome
Biological Process negative regulation of transcription by RNA polymerase II Source:Reactome
Biological Process positive regulation of protein polyubiquitination Source:Ensembl
Biological Process positive regulation of protein ubiquitination Source:UniProtKB1 Publication
Biological Process protein K48-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein polyubiquitination Source:UniProtKB1 Publication
Biological Process ubiquitin-dependent protein catabolic process Source:UniProtKB1 Publication

Cellular Location

Cytoplasm

PTM

Autoubiquitinated in vitro.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant UBE2D1, Active protein, His Tag (CAT#: V2LY-0526-LY7484)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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