Human Recombinant UNC5A protein, ECD, His Tag (V2LY-0526-LY7545)

Name: Human Recombinant UNC5A protein, ECD, His Tag
SKU: V2LY-0526-LY7545
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant UNC5A protein, ECD, His Tag consist of Amino Acid: 1-306 and predicts a molecular mass of 32.7 kDa.

Molecule Mass

32.7 kDa

Protein Domain

ECD

Sequence

Amino Acid: 1-306

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Unc-5 Netrin Receptor A

Function

Receptor for netrin required for axon guidance. Functions in the netrin signaling pathway and promotes neurite outgrowth in response to NTN1. Mediates axon repulsion of neuronal growth cones in the developing nervous system in response to netrin. Axon repulsion in growth cones may be mediated by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand.

Biological Process

Biological Process anterior/posterior axon guidance Source:GO_Central1 Publication
Biological Process apoptotic process Source:UniProtKB-KW
Biological Process axon guidance Source:GO_Central1 Publication
Biological Process netrin-activated signaling pathway Source:UniProtKB
Biological Process neuron projection development Source:UniProtKB

Cellular Location

Cell membrane
Membrane raft
Cell projection, neuron projection
The interaction with PRKCABP regulates its surface expression and leads to its removal from the surface of neurons and growth cones.

Topology

Extracellular: 26-306
Helical: 307-327
Cytoplasmic: 328-842

PTM

Phosphorylated on cytoplasmic tyrosine residues (By similarity).
Phosphorylated by PKC in vitro (By similarity).
Proteolytically cleaved by caspases during apoptosis. The cleavage does not take place when the receptor is associated with netrin ligand. Its cleavage by caspases is required to induce apoptosis.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant UNC5A protein, ECD, hFc Tag (CAT#: V2LY-0526-LY7544)

Related Products

Human Recombinant UNC5A protein, ECD, hFc Tag (CAT#: V2LY-0526-LY7544)

Mouse Anti-UNC5A Recombinant Antibody (CBWJN-1701) (CAT#: CBMAB-N4354-WJ)

Mouse Anti-UNC5A Recombinant Antibody (Dettwy-1) (CAT#: CBMAB-C4661-CN)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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