Human Recombinant USP30 protein, SUMO Tag (V2LY-0526-LY7568)

Name: Human Recombinant USP30 protein, SUMO Tag
SKU: V2LY-0526-LY7568
Rating: 5 (1 reviews)

See all USP30 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

SUMO Tag

Protein Construction

This product is Human Recombinant USP30 protein, SUMO Tag consist of Amino Acid: 48-508 and predicts a molecular mass of 66.3 kDa.

Molecule Mass

66.3 kDa

Sequence

Amino Acid: 48-508

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

ubiquitin specific peptidase 30

Function

Deubiquitinating enzyme tethered to the mitochondrial outer membrane that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PRKN): hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy (PubMed:18287522, PubMed:24896179, PubMed:25527291, PubMed:25621951).
Preferentially cleaves 'Lys-6'- and 'Lys-11'-linked polyubiquitin chains, 2 types of linkage that participate in mitophagic signaling (PubMed:25621951).
Does not cleave efficiently polyubiquitin phosphorylated at 'Ser-65' (PubMed:25527291).
Acts as negative regulator of mitochondrial fusion by mediating deubiquitination of MFN1 and MFN2 (By similarity).

Biological Process

Biological Process autophagy of mitochondrion Source:UniProtKB1 Publication
Biological Process mitochondrial fusion Source:UniProtKB
Biological Process negative regulation of mitophagy Source:Ensembl
Biological Process pexophagy Source:Reactome
Biological Process protein deubiquitination Source:UniProtKB1 Publication
Biological Process protein K11-linked deubiquitination Source:UniProtKB1 Publication
Biological Process protein K6-linked deubiquitination Source:UniProtKB1 Publication
Biological Process ubiquitin-dependent protein catabolic process Source:InterPro

Cellular Location

Mitochondrion outer membrane

Topology

Mitochondrial intermembrane: 1-35
Helical: 36-56
Cytoplasmic: 57-517

PTM

Ubiquitinated by parkin (PRKN) at Lys-235 and Lys-289, leading to its degradation.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human Recombinant USP30 protein, SUMO Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-USP30 Recombinant Antibody (CBXU-013) (CAT#: CBMAB-U0129-CQ)

Mouse Anti-USP30 Recombinant Antibody (CBXU-093) (CAT#: CBMAB-U0192-CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry