Human RFNG ELISA Kit (V2LY-0626-LY2372)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.01 ng/mL
Detection Range
0.02-4.5 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
36.4 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
RFNG O-Fucosylpeptide 3-Beta-N-Acetylglucosaminyltransferase
Function
Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Modulates NOTCH1 activity by modifying O-fucose residues at specific EGF-like domains resulting in enhancement of NOTCH1 activation by DLL1 and JAG1. May be involved in limb formation and in neurogenesis.
Biological Process
Biological Process animal organ morphogenesis1 PublicationNAS:UniProtKB
Biological Process cell differentiationIEA:UniProtKB-KW
Biological Process nervous system developmentIEA:UniProtKB-KW
Biological Process pattern specification processIEA:InterPro
Biological Process positive regulation of Notch signaling pathwayIEA:Ensembl
Biological Process positive regulation of protein bindingIEA:Ensembl
Biological Process regulation of Notch signaling pathwayISS:UniProtKB
Cellular Location
Golgi apparatus membrane
Topology
Cytoplasmic: 1-6
Helical: 7-29
Lumenal: 30-331
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

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Mouse Anti-RFNG Recombinant Antibody (CBCNR-139) (CAT#: CBMAB-R2344-CN)

Mouse Anti-RFNG Recombinant Antibody (9G432) (CAT#: CBMAB-R2343-CN)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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