Human ASPH ELISA Kit (V2LY-0626-LY4178)

Name: Human ASPH ELISA Kit
SKU: V2LY-0626-LY4178
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0012 ng/mL

Detection Range

0.002-0.6 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

85.9 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

aspartate beta-hydroxylase

Function

Isoform 1: specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins.
Isoform 8: membrane-bound Ca2+-sensing protein, which is a structural component of the ER-plasma membrane junctions. Isoform 8 regulates the activity of Ca(+2) released-activated Ca(+2) (CRAC) channels in T-cells.

Biological Process

Activation of cysteine-type endopeptidase activity Source: BHF-UCL
Activation of store-operated calcium channel activity Source: UniProtKB
Calcium ion homeostasis Source: UniProtKB
Calcium ion transmembrane transport Source: UniProtKB
Cellular response to calcium ion Source: UniProtKB
Detection of calcium ion Source: BHF-UCL
Ion transmembrane transport Source: Reactome
Muscle contraction Source: ProtInc
Peptidyl-aspartic acid hydroxylation Source: GO_Central
Positive regulation of calcium ion transport into cytosol Source: UniProtKB
Positive regulation of intracellular protein transport Source: UniProtKB
Positive regulation of proteolysis Source: BHF-UCL
Positive regulation of ryanodine-sensitive calcium-release channel activity Source: BHF-UCL
Positive regulation of transcription, DNA-templated Source: UniProtKB
Regulation of cardiac conduction Source: Reactome
Regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion Source: BHF-UCL
Regulation of cell communication by electrical coupling Source: BHF-UCL
Regulation of inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity Source: UniProtKB
Regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum Source: BHF-UCL
Regulation of ryanodine-sensitive calcium-release channel activity Source: BHF-UCL
Response to ATP Source: UniProtKB

Cellular Location

Isoform 1: Endoplasmic reticulum membrane
Isoform 4: Sarcoplasmic reticulum membrane
Isoform 8: Endoplasmic reticulum membrane

Involvement in disease

Facial dysmorphism, lens dislocation, anterior segment abnormalities, and spontaneous filtering blebs (FDLAB): A syndrome characterized by dislocated crystalline lenses and anterior segment abnormalities in association with a distinctive facies involving flat cheeks and a beaked nose. Some affected individuals develop highly unusual non-traumatic conjunctival cysts (filtering blebs).

Topology

Cytoplasmic: 1-53 aa
Helical: 54-74 aa
Lumenal: 75-758 aa

Nagaoka, K., Ogawa, K., Ji, C., Cao, K. Y., Bai, X., Mulla, J., ... & Huang, C. K. (2021). Targeting aspartate beta-hydroxylase with the small molecule inhibitor MO-I-1182 suppresses cholangiocarcinoma metastasis. Digestive Diseases and Sciences, 66, 1080-1089.

Senthil, S., Sharma, S., Vishwakarma, S., & Kaur, I. (2021). A novel mutation in the aspartate beta-hydroxylase (ASPH) gene is associated with a rare form of Traboulsi syndrome. Ophthalmic Genetics, 42(1), 28-34.

Kanwal, M., Smahel, M., Olsen, M., Smahelova, J., & Tachezy, R. (2020). Aspartate β-hydroxylase as a target for cancer therapy. Journal of Experimental & Clinical Cancer Research, 39(1), 1-12.

Zheng, W., Wang, X., Hu, J., Bai, B., & Zhu, H. (2020). Diverse molecular functions of aspartate β‑hydroxylase in cancer. Oncology Reports.

Benelli, R., Costa, D., Mastracci, L., Grillo, F., Olsen, M. J., Barboro, P., ... & Ferrari, N. (2020). Aspartate-β-hydroxylase: A promising target to limit the local invasiveness of colorectal cancer. Cancers, 12(4), 971.

Barboro, P., Benelli, R., Tosetti, F., Costa, D., Capaia, M., Astigiano, S., ... & Ferrari, N. (2020). Aspartate β-hydroxylase targeting in castration-resistant prostate cancer modulates the NOTCH/HIF1α/GSK3β crosstalk. Carcinogenesis, 41(9), 1246-1252.

Nagaoka, K., Bai, X., Ogawa, K., Dong, X., Zhang, S., Zhou, Y., ... & Wands, J. R. (2019). Anti-tumor activity of antibody drug conjugate targeting aspartate-β-hydroxylase in pancreatic ductal adenocarcinoma. Cancer letters, 449, 87-98.

Holtzman, N. G., Lebowitz, M. S., Koka, R., Baer, M. R., Malhotra, K., Fuller, S. A., ... & Emadi, A. (2018). Aspartate Beta-Hydroxylase (ASPH) As a Novel Therapeutic Target in Acute Myeloid Leukemia. Blood, 132, 5273.

Huang, C. K., Iwagami, Y., Zou, J., Casulli, S., Lu, S., Nagaoka, K., ... & Wands, J. R. (2018). Aspartate beta-hydroxylase promotes cholangiocarcinoma progression by modulating RB1 phosphorylation. Cancer letters, 429, 1-10.

Zou, Q., Hou, Y., Wang, H., Wang, K., Xing, X., Xia, Y., ... & Shen, F. (2018). Hydroxylase activity of ASPH promotes hepatocellular carcinoma metastasis through epithelial-to-mesenchymal transition pathway. EBioMedicine, 31, 287-298.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Asph ELISA Kit (CAT#: V2LY-0626-LY2055)

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Mouse Asph ELISA Kit (CAT#: V2LY-0626-LY2055)

Mouse Anti-ASPH Recombinant Antibody (A10) (CAT#: CBMAB-1152-CN)

Isotype control

For research use only. Not intended for any clinical use.

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