Human SEMG1 ELISA Kit (V2LY-0626-LY3730)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.16 ng/mL
Detection Range
0.3-90 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
52.1 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Semenogelin I
Function
Predominant protein in semen. It participates in the formation of a gel matrix entrapping the accessory gland secretions and ejaculated spermatozoa. Fragments of semenogelin and/or fragments of the related proteins may contribute to the activation of progressive sperm movements as the gel-forming proteins are fragmented by KLK3/PSA.
Alpha-inhibin-92 and alpha-inhibin-31, derived from the proteolytic degradation of semenogelin, inhibit the secretion of pituitary follicle-stimulating hormone.
Biological Process
Biological Process antibacterial humoral responseManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process antimicrobial humoral immune response mediated by antimicrobial peptideManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process coagulationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process inseminationManual Assertion Based On ExperimentTAS:UniProtKB
Biological Process killing of cells of another organismManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of calcium ion importManual Assertion Based On ExperimentIDA:CACAO
Biological Process negative regulation of flagellated sperm motilityManual Assertion Based On ExperimentIDA:CACAO
Biological Process positive regulation of serine-type endopeptidase activityManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process sperm capacitationManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Secreted
PTM
Transglutaminase substrate.
Rapidly cleaved after ejaculation by KLK3/PSA, resulting in liquefaction of the semen coagulum and the progressive release of motile spermatozoa.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-SEMG1 Recombinant Antibody (SGII 6B1/3) (CAT#: CBMAB-S0366-CQ)

Rabbit Anti-SEMG1 Recombinant Antibody (CBXS-1497) (CAT#: CBMAB-S4350-CQ)

Isotype control

For research use only. Not intended for any clinical use.

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