Human SIRT3 ELISA Kit (V2LY-0626-LY3200)
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Basic Information
- Standard Solution: 0.5ml x1
- Pre-coated ELISA Plate: 12 wells * 8 detachable strips
- Standard Diluent: 3ml x1
- Streptavidin-HRP: 6ml x1
- Stop Solution: 6ml x1
- Substrate Solution A: 6ml x1
- Substrate Solution B: 6ml x1
- Wash Buffer Concentrate (25x): 20ml x1
- Biotinylated Antibody: 1ml x1
Formulations & Storage [For reference only, actual COA shall prevail!]
Target
Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues (PubMed:12186850, PubMed:12374852, PubMed:16788062, PubMed:18680753, PubMed:18794531, PubMed:23283301, PubMed:24121500, PubMed:24252090).
Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5PO (PubMed:16788062, PubMed:18680753, PubMed:24121500, PubMed:24252090, PubMed:19535340).
Contributes to the regulation of the cellular energy metabolism (PubMed:24252090).
Important for regulating tissue-specific ATP levels (PubMed:18794531).
In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription (PubMed:23283301).
Acts as a regulator of ceramide metabolism by mediating deacetylation of ceramide synthases CERS1, CERS2 and CERS6, thereby increasing their activity and promoting mitochondrial ceramide accumulation (By similarity).
Biological Process peptidyl-lysine deacetylationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process positive regulation of catalase activityManual Assertion Based On ExperimentIDA:CACAO
Biological Process positive regulation of ceramide biosynthetic processISS:UniProtKB
Biological Process positive regulation of superoxide dismutase activityManual Assertion Based On ExperimentIDA:CACAO
Biological Process protein ADP-ribosylationBy SimilarityISS:GO_Central
Biological Process protein deacetylationManual Assertion Based On ExperimentIDA:UniProtKB
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Please try the standard protocols which include: protocols, troubleshooting and guide.
Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols
Alternative Versions
Mouse Sirt3 ELISA Kit (CAT#: V2LY-0626-LY3239)
Human Recombinant SIRT3, Active protein, GST Tag (CAT#: V2LY-0526-LY6835)
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Human Recombinant SIRT3, Active protein, GST Tag (CAT#: V2LY-0526-LY6835)
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Mouse Anti-SIRT3 Recombinant Antibody (CBT2165) (CAT#: V2LY-0625-LY385)
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Custom Antibody Labeling
We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).
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