Human SLC25A12 ELISA Kit (V2LY-0626-LY5541)

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Tested Data
Request for COA
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Basic Information

Sensitivity
0.0046 ng/mL
Detection Range
0.01-2 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
74.8 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Solute Carrier Family 25 Member 12
Function
Mitochondrial electrogenic aspartate/glutamate antiporter that favors efflux of aspartate and entry of glutamate and proton within the mitochondria as part of the malate-aspartate shuttle (PubMed:11566871, PubMed:19641205, PubMed:24515575).
Also mediates the uptake of L-cysteinesulfinate by mitochondria in exchange of L-glutamate and proton. Can also exchange L-cysteinesulfinate with aspartate in their anionic form without any proton translocation (PubMed:11566871).
Biological Process
Biological Process aspartate family amino acid metabolic processTAS:Reactome
Biological Process aspartate transmembrane transportManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process gluconeogenesisTAS:Reactome
Biological Process glutamate biosynthetic processIEA:Ensembl
Biological Process L-glutamate transmembrane transportManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process malate-aspartate shuttleManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of glucose catabolic process to lactate via pyruvateIEA:Ensembl
Biological Process positive regulation of ATP biosynthetic processIEA:Ensembl
Biological Process positive regulation of glucose metabolic processIEA:Ensembl
Biological Process positive regulation of myelinationIEA:Ensembl
Biological Process response to calcium ionManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Mitochondrion inner membrane
Involvement in disease
Developmental and epileptic encephalopathy 39 (DEE39):
A form of epileptic encephalopathy, a heterogeneous group of severe early-onset epilepsies characterized by refractory seizures, neurodevelopmental impairment, and poor prognosis. Development is normal prior to seizure onset, after which cognitive and motor delays become apparent. DEE39 is characterized by global hypomyelination of the central nervous system, with the gray matter appearing relatively unaffected. Inheritance is autosomal recessive.
Topology
Mitochondrial intermembrane: 2-329
Helical: 330-347
Mitochondrial matrix: 348-390
Helical: 391-410
Mitochondrial intermembrane: 411-433
Helical: 434-447
Mitochondrial matrix: 448-482
Helical: 483-502
Mitochondrial intermembrane: 503-521
Helical: 522-539
Mitochondrial matrix: 540-578
Helical: 579-598
Mitochondrial intermembrane: 599-678
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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