Human ATG12 ELISA Kit (V2LY-0626-LY5413)

Name: Human ATG12 ELISA Kit
SKU: V2LY-0626-LY5413
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0076 ng/mL

Detection Range

0.015-3 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

15.1 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Autophagy Related 12

Function

Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes.
(Microbial infection) May act as a proviral factor. In association with ATG5, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection (PubMed:17709747).
Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for the initiation of HCV replication, but not required once infection is established (PubMed:19666601).

Biological Process

Autophagosome assembly Source: UniProtKB
Autophagy of mitochondrion Source: GO_Central
Autophagy of nucleus Source: GO_Central
C-terminal protein lipidation Source: GO_Central
Macroautophagy Source: Reactome
Viral process Source: UniProtKB-KW

Cellular Location

Cytoplasm; Preautophagosomal structure membrane. TECPR1 recruits the ATG12-ATG5 conjugate to the autolysosomal membrane.

PTM

Acetylated by EP300.

Wu, K., Chen, Y., Wang, D., & He, K. (2021). MicroRNA-520d-3p alleviates hypoxia/reoxygenation-induced damage in human cardiomyocytes by targeting ATG-12. Journal of Thrombosis and Thrombolysis, 1-11.

Li, J., Tong, Y., Zhou, Y., Han, Z., Wang, X., Ding, T., ... & Qiu, C. (2021). LncRNA KCNQ1OT1 as a miR-26a-5p sponge regulates ATG12-mediated cardiomyocyte autophagy and aggravates myocardial infarction. International Journal of Cardiology.

He, H., Song, X., Yang, Z., Mao, Y., Zhang, K., Wang, Y., ... & Li, Y. (2020). Upregulation of KCNQ1OT1 promotes resistance to stereotactic body radiotherapy in lung adenocarcinoma by inducing ATG5/ATG12-mediated autophagy via miR-372-3p. Cell death & disease, 11(10), 1-14.

Karow, M., Fischer, S., Meßling, S., Konertz, R., Riehl, J., Xiong, Q., ... & Eichinger, L. (2020). Functional characterisation of the autophagy ATG12~ 5/16 complex in dictyostelium discoideum. Cells, 9(5), 1179.

Ji, E., Kim, C., Kang, H., Ahn, S., Jung, M., Hong, Y., ... & Lee, E. K. (2019). RNA binding protein HuR promotes autophagosome formation by regulating expression of autophagy-related proteins 5, 12, and 16 in human hepatocellular carcinoma cells. Molecular and cellular biology, 39(6), e00508-18.

Li, Y. B., Yang, T., Wang, J. X., & Zhao, X. F. (2018). The steroid hormone 20-hydroxyecdysone regulates the conjugation of autophagy-related proteins 12 and 5 in a concentration and time-dependent manner to promote insect midgut programmed cell death. Frontiers in endocrinology, 9, 28.

Tan, D., Zhou, C., Han, S., Hou, X., Kang, S., & Zhang, Y. (2018). MicroRNA-378 enhances migration and invasion in cervical cancer by directly targeting autophagy-related protein 12. Molecular medicine reports, 17(5), 6319-6326.

Sun, W. L., Wang, L., Luo, J., Zhu, H. W., & Cai, Z. W. (2018). Ambra1 modulates the sensitivity of breast cancer cells to epirubicin by regulating autophagy via ATG12. Cancer science, 109(10), 3129-3138.

Yang, K., Yu, B., Cheng, C., Cheng, T., Yuan, B., Li, K., ... & Zhou, Y. (2017). Mir505–3p regulates axonal development via inhibiting the autophagy pathway by targeting Atg12. Autophagy, 13(10), 1679-1696.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Atg12 ELISA Kit (CAT#: V2LY-0626-LY3317)

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Isotype control

For research use only. Not intended for any clinical use.

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