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Mouse Anti-ATG12 Recombinant Antibody (1F9) (CBMAB-1158-CN)

This product is a mouse antibody that recognizes ATG12 of human. The antibody 1F9 can be used for immunoassay techniques such as: WB, ELISA.
See all ATG12 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1F9
Antibody Isotype
IgG1
Application
WB, ELISA

Basic Information

Immunogen
ATG12 (AAH12266, aa. 1-140) full-length recombinant protein with GST tag.
Specificity
Human
Antibody Isotype
IgG1
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
Localized in aa. 1-140

Target

Full Name
Autophagy Related 12
Introduction
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy. ATG12 (Autophagy Related 12) is a Protein Coding gene. Among its related pathways are FoxO signaling pathway and Cellular Senescence (REACTOME). GO annotations related to this gene include Atg8 ligase activity. By searching an EST database for sequences similar to yeast Apg12, followed by 5-prime RACE of a HeLa cell cDNA library. The deduced 140-amino acid protein has a calculated molecular mass of 15 kD.
Entrez Gene ID
9140
UniProt ID
O94817
Alternative Names
APG12; FBR93; APG12L; HAPG12
Function
Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes.
(Microbial infection) May act as a proviral factor. In association with ATG5, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection (PubMed:17709747).
Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for the initiation of HCV replication, but not required once infection is established (PubMed:19666601).
Biological Process
Autophagosome assembly Source: UniProtKB
Autophagy of mitochondrion Source: GO_Central
Autophagy of nucleus Source: GO_Central
C-terminal protein lipidation Source: GO_Central
Macroautophagy Source: Reactome
Viral process Source: UniProtKB-KW
Cellular Location
Cytoplasm; Preautophagosomal structure membrane. TECPR1 recruits the ATG12-ATG5 conjugate to the autolysosomal membrane.
PTM
Acetylated by EP300.

Wu, K., Chen, Y., Wang, D., & He, K. (2021). MicroRNA-520d-3p alleviates hypoxia/reoxygenation-induced damage in human cardiomyocytes by targeting ATG-12. Journal of Thrombosis and Thrombolysis, 1-11.

Li, J., Tong, Y., Zhou, Y., Han, Z., Wang, X., Ding, T., ... & Qiu, C. (2021). LncRNA KCNQ1OT1 as a miR-26a-5p sponge regulates ATG12-mediated cardiomyocyte autophagy and aggravates myocardial infarction. International Journal of Cardiology.

He, H., Song, X., Yang, Z., Mao, Y., Zhang, K., Wang, Y., ... & Li, Y. (2020). Upregulation of KCNQ1OT1 promotes resistance to stereotactic body radiotherapy in lung adenocarcinoma by inducing ATG5/ATG12-mediated autophagy via miR-372-3p. Cell death & disease, 11(10), 1-14.

Karow, M., Fischer, S., Meßling, S., Konertz, R., Riehl, J., Xiong, Q., ... & Eichinger, L. (2020). Functional characterisation of the autophagy ATG12~ 5/16 complex in dictyostelium discoideum. Cells, 9(5), 1179.

Ji, E., Kim, C., Kang, H., Ahn, S., Jung, M., Hong, Y., ... & Lee, E. K. (2019). RNA binding protein HuR promotes autophagosome formation by regulating expression of autophagy-related proteins 5, 12, and 16 in human hepatocellular carcinoma cells. Molecular and cellular biology, 39(6), e00508-18.

Li, Y. B., Yang, T., Wang, J. X., & Zhao, X. F. (2018). The steroid hormone 20-hydroxyecdysone regulates the conjugation of autophagy-related proteins 12 and 5 in a concentration and time-dependent manner to promote insect midgut programmed cell death. Frontiers in endocrinology, 9, 28.

Tan, D., Zhou, C., Han, S., Hou, X., Kang, S., & Zhang, Y. (2018). MicroRNA-378 enhances migration and invasion in cervical cancer by directly targeting autophagy-related protein 12. Molecular medicine reports, 17(5), 6319-6326.

Sun, W. L., Wang, L., Luo, J., Zhu, H. W., & Cai, Z. W. (2018). Ambra1 modulates the sensitivity of breast cancer cells to epirubicin by regulating autophagy via ATG12. Cancer science, 109(10), 3129-3138.

Yang, K., Yu, B., Cheng, C., Cheng, T., Yuan, B., Li, K., ... & Zhou, Y. (2017). Mir505–3p regulates axonal development via inhibiting the autophagy pathway by targeting Atg12. Autophagy, 13(10), 1679-1696.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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