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Rabbit Anti-ATG12 Recombinant Antibody (A3894) (CBMAB-A3894-YC)

Provided herein is a Rabbit monoclonal antibody against Human Autophagy Related 12. The antibody can be used for immunoassay techniques, such as FC, ICC, IF, WB.
See all ATG12 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
A3894
Antibody Isotype
IgG
Application
ICC, IF, WB

Basic Information

Immunogen
Peptides corresponding to Human ATG12 (aa 43-57, 122-133).
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1-2 µg/ml
IF(ICC)2 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Preservative
0.09% sodium azide
Concentration
0.5 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Autophagy Related 12
Introduction
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog o
Entrez Gene ID
Human9140
Mouse67526
UniProt ID
HumanO94817
MouseQ9CQY1
Alternative Names
Autophagy Related 12; APG12 Autophagy 12-Like; APG12L; APG12; ATG12 Autophagy Related 12 Homolog (S. Cerevisiae); Apg12 (Autophagy 12, S. Cerevisiae)-Like; APG12 Autophagy 12-Like (S. Cerevisiae); ATG12 Autophagy Related 12 Homolog;
Function
Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes.
(Microbial infection) May act as a proviral factor. In association with ATG5, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection (PubMed:17709747).
Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for the initiation of HCV replication, but not required once infection is established (PubMed:19666601).
Biological Process
Autophagosome assembly Source: UniProtKB
Autophagy of mitochondrion Source: GO_Central
Autophagy of nucleus Source: GO_Central
C-terminal protein lipidation Source: GO_Central
Macroautophagy Source: Reactome
Viral process Source: UniProtKB-KW
Cellular Location
Cytoplasm; Preautophagosomal structure membrane. TECPR1 recruits the ATG12-ATG5 conjugate to the autolysosomal membrane.
PTM
Acetylated by EP300.

Wu, K., Chen, Y., Wang, D., & He, K. (2021). MicroRNA-520d-3p alleviates hypoxia/reoxygenation-induced damage in human cardiomyocytes by targeting ATG-12. Journal of Thrombosis and Thrombolysis, 1-11.

Li, J., Tong, Y., Zhou, Y., Han, Z., Wang, X., Ding, T., ... & Qiu, C. (2021). LncRNA KCNQ1OT1 as a miR-26a-5p sponge regulates ATG12-mediated cardiomyocyte autophagy and aggravates myocardial infarction. International Journal of Cardiology.

He, H., Song, X., Yang, Z., Mao, Y., Zhang, K., Wang, Y., ... & Li, Y. (2020). Upregulation of KCNQ1OT1 promotes resistance to stereotactic body radiotherapy in lung adenocarcinoma by inducing ATG5/ATG12-mediated autophagy via miR-372-3p. Cell death & disease, 11(10), 1-14.

Karow, M., Fischer, S., Meßling, S., Konertz, R., Riehl, J., Xiong, Q., ... & Eichinger, L. (2020). Functional characterisation of the autophagy ATG12~ 5/16 complex in dictyostelium discoideum. Cells, 9(5), 1179.

Ji, E., Kim, C., Kang, H., Ahn, S., Jung, M., Hong, Y., ... & Lee, E. K. (2019). RNA binding protein HuR promotes autophagosome formation by regulating expression of autophagy-related proteins 5, 12, and 16 in human hepatocellular carcinoma cells. Molecular and cellular biology, 39(6), e00508-18.

Li, Y. B., Yang, T., Wang, J. X., & Zhao, X. F. (2018). The steroid hormone 20-hydroxyecdysone regulates the conjugation of autophagy-related proteins 12 and 5 in a concentration and time-dependent manner to promote insect midgut programmed cell death. Frontiers in endocrinology, 9, 28.

Tan, D., Zhou, C., Han, S., Hou, X., Kang, S., & Zhang, Y. (2018). MicroRNA-378 enhances migration and invasion in cervical cancer by directly targeting autophagy-related protein 12. Molecular medicine reports, 17(5), 6319-6326.

Sun, W. L., Wang, L., Luo, J., Zhu, H. W., & Cai, Z. W. (2018). Ambra1 modulates the sensitivity of breast cancer cells to epirubicin by regulating autophagy via ATG12. Cancer science, 109(10), 3129-3138.

Yang, K., Yu, B., Cheng, C., Cheng, T., Yuan, B., Li, K., ... & Zhou, Y. (2017). Mir505–3p regulates axonal development via inhibiting the autophagy pathway by targeting Atg12. Autophagy, 13(10), 1679-1696.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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