Human SMC3 ELISA Kit (V2LY-0626-LY3863)

Name: Human SMC3 ELISA Kit
SKU: V2LY-0626-LY3863
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.026 ng/mL

Detection Range

0.05-15 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

141.5 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

structural maintenance of chromosomes 3

Function

Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.

Biological Process

Cell division
DNA repair
Meiotic cell cycle
Mitotic cell cycle
Mitotic spindle assembly
Regulation of DNA replication
Sister chromatid cohesion
Stem cell population maintenance

Cellular Location

Nucleus; Chromosome; Centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. The phosphorylated form at Ser-1083 is preferentially associated with unsynapsed chromosomal regions (By similarity).

Involvement in disease

A form of Cornelia de Lange syndrome, a clinically heterogeneous developmental disorder associated with malformations affecting multiple systems. Characterized by facial dysmorphisms, abnormal hands and feet, growth delay, cognitive retardation, hirsutism, gastroesophageal dysfunction and cardiac, ophthalmologic and genitourinary anomalies. Cornelia de Lange syndrome type 3 is a mild form with absence of major structural anomalies. The phenotype in some instances approaches that of apparently non-syndromic mental retardation.

PTM

Ubiquitinated by the DCX(DCAF15) complex, leading to its degradation.
Phosphorylated at Ser-1083 in a SPO11-dependent manner.
Acetylation at Lys-105 and Lys-106 by ESCO1 is important for genome stability and S phase sister chromatid cohesion. Regulated by DSCC1, it is required for processive DNA synthesis, coupling sister chromatid cohesion establishment during S phase to DNA replication. Deacetylation by HDAC8, regulates release of the cohesin complex from chromatin.

Rivas, M. A., Meydan, C., Chin, C. R., Challman, M. F., Kim, D., Bhinder, B., ... & Melnick, A. M. (2021). Smc3 dosage regulates B cell transit through germinal centers and restricts their malignant transformation. Nature Immunology, 22(2), 240-253.

Reichmann, J., Dobie, K., Lister, L. M., Crichton, J. H., Best, D., MacLennan, M., ... & Adams, I. R. (2020). Tex19. 1 inhibits the N-end rule pathway and maintains acetylated SMC3 cohesin and sister chromatid cohesion in oocytes. Journal of Cell Biology, 219(5).

Wang, N., Zhu, Y., Xie, M., Wang, L., Jin, F., Li, Y., ... & De, W. (2018). Long noncoding RNA Meg3 regulates mafa expression in mouse beta cells by inactivating Rad21, Smc3 or Sin3α. Cellular Physiology and Biochemistry, 45(5), 2031-2043.

Robison, B., Guacci, V., & Koshland, D. (2018). A role for the Smc3 hinge domain in the maintenance of sister chromatid cohesion. Molecular biology of the cell, 29(3), 339-355.

Wang, D., Wang, L., Zhang, Y., Zhao, Y., & Chen, G. (2018). Hydrogen gas inhibits lung cancer progression through targeting SMC3. Biomedicine & Pharmacotherapy, 104, 788-797.

Infante, E., Alkorta‐Aranburu, G., & El‐Gharbawy, A. (2017). Rare form of autosomal dominant familial Cornelia de Lange syndrome due to a novel duplication in SMC3. Clinical case reports, 5(8), 1277.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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