Human SOAT1 ELISA Kit (V2LY-0626-LY2933)

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Tested Data
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Basic Information

Sensitivity
0.12 ng/mL
Detection Range
0.2-60 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
64.7 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Sterol O-Acyltransferase 1
Function
Catalyzes the formation of fatty acid-cholesterol esters, which are less soluble in membranes than cholesterol (PubMed:16154994, PubMed:16647063, PubMed:9020103, PubMed:32433614, PubMed:32433613, PubMed:32944968).
Plays a role in lipoprotein assembly and dietary cholesterol absorption (PubMed:16154994, PubMed:9020103).
Utilizes oleoyl-CoA ((9Z)-octadecenoyl-CoA) preferentially as susbstrate: shows a higher activity towards an acyl-CoA substrate with a double bond at the delta-9 position (9Z) than towards saturated acyl-CoA or an unsaturated acyl-CoA with a double bond at the delta-7 (7Z) or delta-11 (11Z) positions (PubMed:11294643, PubMed:32433614).
Biological Process
Biological Process cholesterol effluxManual Assertion Based On ExperimentIMP:BHF-UCL
Biological Process cholesterol esterificationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process cholesterol homeostasisManual Assertion Based On ExperimentTAS:BHF-UCL
Biological Process cholesterol metabolic processManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process cholesterol storageManual Assertion Based On ExperimentIMP:BHF-UCL
Biological Process low-density lipoprotein particle clearanceTAS:Reactome
Biological Process macrophage derived foam cell differentiationManual Assertion Based On ExperimentIMP:BHF-UCL
Biological Process positive regulation of amyloid precursor protein biosynthetic processManual Assertion Based On ExperimentIMP:BHF-UCL
Biological Process very-low-density lipoprotein particle assemblyManual Assertion Based On ExperimentIMP:BHF-UCL
Cellular Location
Endoplasmic reticulum membrane
Topology
Cytoplasmic: 1-138
Helical: 139-160
Lumenal: 161-180
Helical: 181-206
Cytoplasmic: 207-218
Helical: 219-244
Lumenal: 245-252
Helical: 253-276
Cytoplasmic: 277-319
Helical: 320-352
Lumenal: 353-369
Helical: 370-395
Cytoplasmic: 396-443
Helical: 444-468
Lumenal: 469-474
Helical: 475-490
Cytoplasmic: 491-496
Helical: 497-528
Lumenal: 529-550
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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