Mouse Anti-5-hmC Recombinant Antibody (V2-7668) (CBMAB-1840CQ)

Name: Mouse Anti-5-hmC Recombinant Antibody (V2-7668)
SKU: CBMAB-1840CQ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-5-hmC Recombinant Antibody (13612) in Dot blot

5-hmC and 5-mC levels in pancreatic tumor tissues verses adjacent non-tumor tissues were detected by dot blot. ...View More

Wu, J., Li, H., Shi, M., Zhu, Y., Ma, Y., Zhong, Y., ... & Peng, C. (2019). TET1-mediated DNA hydroxymethylation activates inhibitors of the Wnt/β-catenin signaling pathway to suppress EMT in pancreatic tumor cells. Journal of Experimental & Clinical Cancer Research, 38, 1-17.

Mouse Anti-5-hmC Recombinant Antibody (13612) in IHC

A, B Hematoxylin and eosin staining of gastric adenocarcinoma with enteroblastic differentiation (GAED) tissue. Preserved Teneleven translocation 1 (TET1) expression was observed in a case of GAED (C: corresponding to
A). Reduced TET1 expression was observed in a case of GAED (D: corresponding to B). Preserved 5-hydroxymethylcytosine (5-hmc) expression was observed in a case of GAED (E: corresponding to A). Reduced 5-hmc expression
was observed in a case of GAED (F: corresponding to B). Original magnification (A-D), 200× ...View More

Yatagai, N., Saito, T., Akazawa, Y., Hayashi, T., Yanai, Y., Tsuyama, S., ... & Yao, T. (2019). TP53 inactivation and expression of methylation-associated proteins in gastric adenocarcinoma with enteroblastic differentiation. Virchows Archiv, 474, 315-324.

Mouse Anti-5-hmC Recombinant Antibody (13612) in Dot blot

5hmC levels in MDS-L cells transduced with shSIRT1 #1, shSIRT1 #2 or shCtrl were determined by dot blot. ...View More

Sun, J., He, X., Zhu, Y., Ding, Z., Dong, H., Feng, Y., ... & Li, L. (2018). SIRT1 activation disrupts maintenance of myelodysplastic syndrome stem and progenitor cells by restoring TET2 function. Cell stem cell, 23(3), 355-369.

Datasheet

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Basic Information

Host Animal

Mouse

Clone

V2-7668

Application

IHC-P, IHC, DB, MD

Immunogen

5-HydroxymethylCytosine / 5-hmC.

Host Species

Mouse

Antibody Isotype

IgG2b

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC-P	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

5-Hydroxymethylcytosine

Introduction

5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base derived from cytosine. It is potentially important in epigenetics, because the hydroxymethyl group on the cytosine can possibly switch a gene on and off.

Alternative Names

5-Hydroxymethylcytosine; 1123-95-1; 4-amino-5-(hydroxymethyl)pyrimidin-2-ol; 4-Amino-5-(hydroxymethyl)-2(1H)-pyrimidinone; 5-HYDROXYMETHYL CYTOSINE; 6-amino-5-(hydroxymethyl)-1H-pyrimidin-2-one; 5-hmc; 5hmc; C5H7N3O2

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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