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Rabbit Anti-5-hmC Recombinant Antibody (V2-7944) (CBMAB-2134CQ)


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Published Data
Rabbit Anti-5-hmC Recombinant Antibody (RM236) in ELISA
Rabbit Anti-5-hmC Recombinant Antibody (RM236) in ELISA
Calibration curves of the ELISA for 5hmC. The UV absorbance at 450 nm is plotted against the ratio of 5hmC in all the DNA bases (mean ± SD. n = 4). ...View More
Kojima, N., Suda, T., Fujii, S., Hirano, K., Namihira, M., & Kurita, R. (2020). Quantitative analysis of global 5-methyl-and 5-hydroxymethylcytosine in TET1 expressed HEK293T cells. Biosensors and Bioelectronics, 167, 112472.

Summary

Host Animal
Rabbit
Specificity
Chemical
Clone
V2-7944
Antibody Isotype
IgG
Application
DB, ELISA, IF, IHC, IHC-P, MD

Basic Information

Immunogen
BSA-conjugated 5-hydroxymethylcytosine
Host Species
Rabbit
Specificity
Chemical
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
DB0.2-1 µg/ml
ELISA0.1-1 µg/ml
IHC-P0.1-1 µg/ml
IF(ICC)0.5-2 µg/ml
FC1-5 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 1% BSA, 50% Glycerol
Preservative
0.09% sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
5-Hydroxymethylcytosine
Introduction
5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base derived from cytosine. It is potentially important in epigenetics, because the hydroxymethyl group on the cytosine can possibly switch a gene on and off.
Alternative Names
5-Hydroxymethylcytosine; 1123-95-1; 4-amino-5-(hydroxymethyl)pyrimidin-2-ol; 4-Amino-5-(hydroxymethyl)-2(1H)-pyrimidinone; 5-HYDROXYMETHYL CYTOSINE; 6-amino-5-(hydroxymethyl)-1H-pyrimidin-2-one; 5-hmc; 5hmc; C5H7N3O2
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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