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Rat Anti-5-hmC Recombinant Antibody (V2-7757) (CBMAB-1938CQ)


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Published Data
Rat Anti-5-hmC Recombinant Antibody (AB3/63.3) in Dot blot
Rat Anti-5-hmC Recombinant Antibody (AB3/63.3) in Dot blot
The specificities of the antibodies used in this study were confirmed by dot blot and (h)MeDIP using PCR fragments containing 5hmC, 5mC or C. ...View More
Ficz, G., Branco, M. R., Seisenberger, S., Santos, F., Krueger, F., Hore, T. A., ... & Reik, W. (2011). Dynamic regulation of 5-hydroxymethylcytosine in mouse ES cells and during differentiation. Nature, 473(7347), 398-402.
Rat Anti-5-hmC Recombinant Antibody (AB3/63.3) in IF
Rat Anti-5-hmC Recombinant Antibody (AB3/63.3) in IF
Immunofluorescence co-staining of J1 ES cells with antibodies against 5hmC (green) and 5mC (red). Grey scale images of the two modifications are shown separately. Staining for 5mC is particularly strong in pericentromeric heterochromatin (arrows), contrary to 5hmC. Scale bar, 10 mm ...View More
Ficz, G., Branco, M. R., Seisenberger, S., Santos, F., Krueger, F., Hore, T. A., ... & Reik, W. (2011). Dynamic regulation of 5-hydroxymethylcytosine in mouse ES cells and during differentiation. Nature, 473(7347), 398-402.

Summary

Host Animal
Rat
Clone
V2-7757
Antibody Isotype
IgG2a, κ
Application
IHC-P, CI, DB, IF, MD

Basic Information

Immunogen
5-Hydroxymethylcytosine conjugated to BSA
Host Species
Rat
Antibody Isotype
IgG2a, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.02% sodium azide
Concentration
1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
5-Hydroxymethylcytosine
Introduction
5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base derived from cytosine. It is potentially important in epigenetics, because the hydroxymethyl group on the cytosine can possibly switch a gene on and off.
Alternative Names
5-Hydroxymethylcytosine; 1123-95-1; 4-amino-5-(hydroxymethyl)pyrimidin-2-ol; 4-Amino-5-(hydroxymethyl)-2(1H)-pyrimidinone; 5-HYDROXYMETHYL CYTOSINE; 6-amino-5-(hydroxymethyl)-1H-pyrimidin-2-one; 5-hmc; 5hmc; C5H7N3O2
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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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