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Mouse Anti-AKAP12 Recombinant Antibody (V2-180433) (CBMAB-A1910-YC)

Provided herein is a Mouse monoclonal antibody against Human A-Kinase Anchoring Protein 12. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all AKAP12 antibodies
Published Data
Fig.1 TMAs and western blot validation analysis of AKAP12, DCBLD2, NT5E and SPON1.
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Fig.2 Four representative frozen CRC specimens (T) and matched normal mucosa (N) were identified in the same cohort of patients and analyzed by immunoblot.
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Fig.3 Western blots of proteins identified to have differential expression from iTRAQ data.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-180433
Antibody Isotype
IgG2b, κ
Application
ELISA, WB

Basic Information

Immunogen
Partial recombinant corresponding to aa1675-1782 from AKAP12 (NP_005091) with GST tag.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
A-Kinase Anchoring Protein 12
Introduction
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene
Entrez Gene ID
9590
UniProt ID
Q02952
Alternative Names
A-Kinase Anchoring Protein 12; Src-Suppressed C Kinase Substrate; A Kinase (PRKA) Anchor Protein 12; AKAP 250; AKAP250; Gravin; A Kinase (PRKA) Anchor Protein (Gravin) 12; Myasthenia Gravis Autoantigen Gravin;
Function
Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).
Biological Process
Adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway Source: UniProtKB
Cellular response to interleukin-1 Source: Ensembl
Cellular response to tumor necrosis factor Source: Ensembl
G protein-coupled receptor signaling pathway Source: ProtInc
Hepatic stellate cell activation Source: Ensembl
Modulation of chemical synaptic transmission Source: Ensembl
Negative regulation of vascular permeability Source: Ensembl
Positive regulation of ERK1 and ERK2 cascade Source: Ensembl
Positive regulation of hepatic stellate cell migration Source: Ensembl
Positive regulation of nitric-oxide synthase biosynthetic process Source: Ensembl
Positive regulation of oligodendrocyte apoptotic process Source: Ensembl
Positive regulation of protein kinase A signaling Source: InterPro
Regulation of protein kinase A signaling Source: UniProtKB
Regulation of protein kinase C signaling Source: InterPro
Response to electrical stimulus Source: Ensembl
Response to lipopolysaccharide Source: Ensembl
Cellular Location
Cytoskeleton; Cell cortex; Membrane. May be part of the cortical cytoskeleton.

Bai, Y., Yuan, R., Luo, Y., Kang, Z., Zhu, H., Qu, L., ... & Song, X. (2021). Exploration of Genetic Variants within the Goat A-Kinase Anchoring Protein 12 (AKAP12) Gene and Their Effects on Growth Traits. Animals, 11(7), 2090.

Mamoor, S. (2021). Differential expression of A-kinase anchoring protein 12 in cancers of the breast.

Fonseca-Camarillo, G., Furuzawa-Carballeda, J., Priego-Ranero, Á. A., Zúñiga, R. B., Martínez-Benítez, B., & Yamamoto-Furusho, J. K. (2021). AKAP12/Gravin is over-expressed in patients with ulcerative colitis. Immunologic Research, 1-7.

Çiçek, H., Saygılı, Ö., Sever, Ö. N., Kaya, V., Ulusal, H., & Yıldırım, M. (2019). The diagnostic role of A-kinase anchoring protein 12, Bcl-2 and high mobility group box Protein-1 levels in breast cancer. Journal of Oncological Sciences, 5(3), 90-95.

Ibarrola Ulzurrun, J. F., Sádaba Sagredo, R., Martínez Martínez, E., García Peña, A., & Gaínza Calleja, A. (2018). Aldosterone impairs mitochondrial function in human cardiac fibroblasts via A-kinase anchor protein 12. Scientific Reports, 2018, 8, 6801.

Lee, H. S., Choi, J., Son, T., Lee, E. J., Kim, J. G., Ryu, S. H., ... & Kim, K. W. (2018). A‑kinase anchoring protein 12 is downregulated in human hepatocellular carcinoma and its deficiency in mice aggravates thioacetamide‑induced liver injury. Oncology letters, 16(5), 5907-5915.

Ibarrola, J., Sadaba, R., Martinez-Martinez, E., Garcia-Peña, A., Arrieta, V., Alvarez, V., ... & Lopez-Andres, N. (2018). Aldosterone impairs mitochondrial function in human cardiac fibroblasts via A-kinase anchor protein 12. Scientific reports, 8(1), 1-11.

Selvaraju, V., Suresh, S. C., Thirunavukkarasu, M., Mannu, J., Foye, J. L., Mathur, P. P., ... & Maulik, N. (2017). Regulation of A-kinase-anchoring protein 12 by heat shock protein A12B to prevent ventricular dysfunction following acute myocardial infarction in diabetic rats. Journal of cardiovascular translational research, 10(2), 209-220.

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For research use only. Not intended for any clinical use.

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