Mouse Anti-AKR1B10 Recombinant Antibody (V2-180469) (CBMAB-A1977-YC)

Name: Mouse Anti-AKR1B10 Recombinant Antibody (V2-180469)
SKU: CBMAB-A1977-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-AKR1B10 Recombinant Antibody (1A6) in IHC

Immunohistochemistry and histopathological evaluation reveal AKR1B10 expression and specific morphological features in FH-proficient leiomyomas.
a 2SC and AKR1B10 antibodies can both act as biomarkers for FH-deficiency. Immunohistochemistry showed strong staining with both antibodies in FH-deficient tumors. It also revealed a subgroup of tumors positive only for AKR1B10 (AKR1B10hi). Histopathological evaluation of the AKR1B10hi tumors showed increased cellularity in most of them. Myometrium was negative for both AKR1B10 and 2SC. b Half of the AKR1B10hi tumors showed two or more features associated with FH-deficiency [37], including eosinophilic inclusions (top left), staghorn vasculature (top right), eosinophilic nucleoli and nuclear atypia (bottom left), and alveolar edema (bottom right). Example of eosinophilic nucleoli is marked with an arrow. c Half of the AKR1B10hi samples displayed perivascular hypercellularity, a feature not associated with leiomyomas previously. Magnification 100× (left) and 200× (right). ...View More

Mehine, M., Ahvenainen, T., Khamaiseh, S., Härkönen, J., Reinikka, S., Heikkinen, T., ... & Vahteristo, P. (2022). A novel uterine leiomyoma subtype exhibits NRF2 activation and mutations in genes associated with neddylation of the Cullin 3-RING E3 ligase. Oncogenesis, 11(1), 52.

Mouse Anti-AKR1B10 Recombinant Antibody (1A6) in IHC

Light photomicrographs of a haematoxylin and eosin-stained section of a conventional chordoma (top panel) and a chordoma showing immunoreactivity for AKR1B10 (bottom panel). Scale bar = 200 μm. Inset: positive control, normal colonic tissue. ...View More

Nelson, A. C., Pillay, N., Henderson, S., Presneau, N., Tirabosco, R., Halai, D., ... & Flanagan, A. M. (2012). An integrated functional genomics approach identifies the regulatory network directed by brachyury (T) in chordoma. The Journal of pathology, 228(3), 274-285.

Mouse Anti-AKR1B10 Recombinant Antibody (1A6) in IHC

Immunohistochemical analysis of AKR1B10 expression in human pancreatic tissues: (A) No detectable expression was observed in normal pancreatic acini or ducts. (B) Well-differentiated pancreatic ductal adenocarcinoma with high expression of AKR1B10, but negligible expression in an adjacent normal duct (open black arrow). (C) Precancerous pancreatic epithelial neoplasia also over-expressed AKR1B10 as demonstrated in pancreatic intraepithelial neoplasia-3 (large duct in center) and pancreatic intraepithelial neoplasia-1 lesions (open black arrows). ...View More

Chung, Y. T., Matkowskyj, K. A., Li, H., Bai, H., Zhang, W., Tsao, M. S., ... & Yang, G. Y. (2012). Overexpression and oncogenic function of aldo-keto reductase family 1B10 (AKR1B10) in pancreatic carcinoma. Modern pathology, 25(5), 758-766.

Mouse Anti-AKR1B10 Recombinant Antibody (1A6) in WB

Physiological concentrations of luteolin increased the expression of phase II drug-metabolizing enzymes. HepG2 cells were treated at the indicated concentrations of luteolin for 24 h. Cell lysate was used to detect GSTA1, M1 and P1, NQO1, HO-1, AKR1B10 and β-actin by western blot analysis with their respective antibodies (top panels). The intensity of each band was quantified by ImageJ 1.44 and the relative amount of each protein was obtained by normalizing it to the β-actin level and expressing it as a ratio (bottom panels). The results are represented as the mean ± SD (n = 3). Asterisks indicate a significant difference from the corresponding positive control by Dunnett's test (p < 0.05). ...View More

Kitakaze, T., Makiyama, A., Samukawa, Y., Jiang, S., Yamashita, Y., & Ashida, H. (2019). A physiological concentration of luteolin induces phase II drug-metabolizing enzymes through the ERK1/2 signaling pathway in HepG2 cells. Archives of biochemistry and biophysics, 663, 151-159.

Mouse Anti-AKR1B10 Recombinant Antibody (1A6) in WB

Cells were harvested after 24 h and the relative luciferase activity was measured. Cells were transfected with control EV or a vector expressing Flag-Keap1 and harvested for Western blot to examine the protein and mRNA expression levels of Keap1, Nrf2, and ARE-containing Nrf2 target genes. ...View More

Park, S. H., Kim, J. H., Ko, E., Kim, J. Y., Park, M. J., Kim, M. J., ... & Lee, J. Y. (2018). Resistance to gefitinib and cross-resistance to irreversible EGFR-TKIs mediated by disruption of the Keap1-Nrf2 pathway in human lung cancer cells. The FASEB Journal, 32(11), 5862-5873.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-180469

Application

ELISA, ICC, IF, IHC-P, WB

Immunogen

AKR1B10 (NP_064695, 76 a.a. ~ 143 a.a) partial recombinant protein with GST tag.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IF(ICC)	10 μg/ml
IHC-P	3 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Aldo-Keto Reductase Family 1 Member B10

Introduction

AKR1B10 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member can efficiently reduce aliphatic and aromatic aldehydes, and it is less active on hexoses. It is highly expressed in adrenal

Entrez Gene ID

57016

UniProt ID

O60218

Alternative Names

Aldo-Keto Reductase Family 1 Member B10; Aldose Reductase-Related Protein; Small Intestine Reductase; Aldo-Keto Reductase Family 1, Member B11 (Aldose Reductase-Like); Aldo-Keto Reductase Family 1, Member B10 (Aldose Reductase); Aldose Reductase-Like Pept

Function

Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols (PubMed:9565553, PubMed:18087047, PubMed:12732097, PubMed:19013440, PubMed:19563777). Displays strong enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal (PubMed:12732097, PubMed:18087047). Plays a critical role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls) (PubMed:19013440, PubMed:19563777). Displays no reductase activity towards glucose (PubMed:12732097).

Biological Process

Cellular detoxification of aldehyde Source: UniProtKB
Daunorubicin metabolic process Source: UniProtKB
Doxorubicin metabolic process Source: UniProtKB
Farnesol catabolic process Source: UniProtKB
Retinoid metabolic process Source: UniProtKB

Cellular Location

Lysosome; Secreted. Secreted through a lysosome-mediated non-classical pathway.

Liu, R., Zheng, S., yan Yang, C., Yu, Y., Peng, S., Ge, Q., ... & Shao, Y. (2021). Prognostic value of aldo-keto reductase family 1 member B10 (AKR1B10) in digestive system cancers: A meta-analysis. Medicine, 100(14).

Chen, D., Shen, Z., Cheng, X., Wang, Q., Zhou, J., Ren, F., ... & Huang, R. (2021). Homeobox A5 activates p53 pathway to inhibit proliferation and promote apoptosis of adrenocortical carcinoma cells by inducing Aldo-Keto reductase family 1 member B10 expression. Bioengineered, 12(1), 1964-1975.

Kanno, M., Kawaguchi, K., Honda, M., Horii, R., Takatori, H., Shimakami, T., ... & Kaneko, S. (2019). Serum aldo–keto reductase family 1 member B10 predicts advanced liver fibrosis and fatal complications of nonalcoholic steatohepatitis. Journal of gastroenterology, 54(6), 549-557.

Ye, X., Li, C., Zu, X., Lin, M., Liu, Q., Liu, J., ... & Cao, D. (2019). A large‐scale multicenter study validates aldo‐keto reductase family 1 member B10 as a prevalent serum marker for detection of hepatocellular carcinoma. Hepatology, 69(6), 2489-2501.

Shi, J., Chen, L., Chen, Y., Lu, Y., Chen, X., & Yang, Z. (2019). Aldo-Keto Reductase Family 1 Member B10 (AKR1B10) overexpression in tumors predicts worse overall survival in hepatocellular carcinoma. Journal of Cancer, 10(20), 4892.

Liu, W., Song, J., Du, X., Zhou, Y., Li, Y., Li, R., ... & Wang, Q. (2019). AKR1B10 (Aldo-keto reductase family 1 B10) promotes brain metastasis of lung cancer cells in a multi-organ microfluidic chip model. Acta biomaterialia, 91, 195-208.

Han, C., Gao, L., Zhao, L., Sheng, Q., Zhang, C., An, Z., ... & Dou, X. (2018). Immunohistochemistry detects increased expression of aldo-keto reductase family 1 member b10 (AKR1B10) in early-stage hepatocellular carcinoma. Medical science monitor: international medical journal of experimental and clinical research, 24, 7414.

Han, C., Gao, L., Bai, H., & Dou, X. (2018). Identification of a role for serum aldo‑keto reductase family 1 member B10 in early detection of hepatocellular carcinoma. Oncology Letters, 16(6), 7123-7130.

Zhou, Z., Zhao, Y., Gu, L., Niu, X., & Lu, S. (2018). Inhibiting proliferation and migration of lung cancer using small interfering RNA targeting on Aldo-keto reductase family 1 member B10. Molecular medicine reports, 17(2), 2153-2160.

Mori, M., Genda, T., Ichida, T., Murata, A., Kamei, M., Tsuzura, H., ... & Watanabe, S. (2017). Aldo‐keto reductase family 1 member B10 is associated with hepatitis B virus‐related hepatocellular carcinoma risk. Hepatology Research, 47(3), E85-E93.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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