Human AKR1B10 ELISA Kit (V2LY-0626-LY3803)

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Tested Data
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.021 ng/mL
Detection Range
0.05-10 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
36.0 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Aldo-Keto Reductase Family 1 Member B10
Function
Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols (PubMed:9565553, PubMed:18087047, PubMed:12732097, PubMed:19013440, PubMed:19563777). Displays strong enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal (PubMed:12732097, PubMed:18087047). Plays a critical role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls) (PubMed:19013440, PubMed:19563777). Displays no reductase activity towards glucose (PubMed:12732097).
Biological Process
Cellular detoxification of aldehyde Source: UniProtKB
Daunorubicin metabolic process Source: UniProtKB
Doxorubicin metabolic process Source: UniProtKB
Farnesol catabolic process Source: UniProtKB
Retinoid metabolic process Source: UniProtKB
Cellular Location
Lysosome; Secreted. Secreted through a lysosome-mediated non-classical pathway.

Liu, R., Zheng, S., yan Yang, C., Yu, Y., Peng, S., Ge, Q., ... & Shao, Y. (2021). Prognostic value of aldo-keto reductase family 1 member B10 (AKR1B10) in digestive system cancers: A meta-analysis. Medicine, 100(14).

Chen, D., Shen, Z., Cheng, X., Wang, Q., Zhou, J., Ren, F., ... & Huang, R. (2021). Homeobox A5 activates p53 pathway to inhibit proliferation and promote apoptosis of adrenocortical carcinoma cells by inducing Aldo-Keto reductase family 1 member B10 expression. Bioengineered, 12(1), 1964-1975.

Kanno, M., Kawaguchi, K., Honda, M., Horii, R., Takatori, H., Shimakami, T., ... & Kaneko, S. (2019). Serum aldo–keto reductase family 1 member B10 predicts advanced liver fibrosis and fatal complications of nonalcoholic steatohepatitis. Journal of gastroenterology, 54(6), 549-557.

Ye, X., Li, C., Zu, X., Lin, M., Liu, Q., Liu, J., ... & Cao, D. (2019). A large‐scale multicenter study validates aldo‐keto reductase family 1 member B10 as a prevalent serum marker for detection of hepatocellular carcinoma. Hepatology, 69(6), 2489-2501.

Shi, J., Chen, L., Chen, Y., Lu, Y., Chen, X., & Yang, Z. (2019). Aldo-Keto Reductase Family 1 Member B10 (AKR1B10) overexpression in tumors predicts worse overall survival in hepatocellular carcinoma. Journal of Cancer, 10(20), 4892.

Liu, W., Song, J., Du, X., Zhou, Y., Li, Y., Li, R., ... & Wang, Q. (2019). AKR1B10 (Aldo-keto reductase family 1 B10) promotes brain metastasis of lung cancer cells in a multi-organ microfluidic chip model. Acta biomaterialia, 91, 195-208.

Han, C., Gao, L., Zhao, L., Sheng, Q., Zhang, C., An, Z., ... & Dou, X. (2018). Immunohistochemistry detects increased expression of aldo-keto reductase family 1 member b10 (AKR1B10) in early-stage hepatocellular carcinoma. Medical science monitor: international medical journal of experimental and clinical research, 24, 7414.

Han, C., Gao, L., Bai, H., & Dou, X. (2018). Identification of a role for serum aldo‑keto reductase family 1 member B10 in early detection of hepatocellular carcinoma. Oncology Letters, 16(6), 7123-7130.

Zhou, Z., Zhao, Y., Gu, L., Niu, X., & Lu, S. (2018). Inhibiting proliferation and migration of lung cancer using small interfering RNA targeting on Aldo-keto reductase family 1 member B10. Molecular medicine reports, 17(2), 2153-2160.

Mori, M., Genda, T., Ichida, T., Murata, A., Kamei, M., Tsuzura, H., ... & Watanabe, S. (2017). Aldo‐keto reductase family 1 member B10 is associated with hepatitis B virus‐related hepatocellular carcinoma risk. Hepatology Research, 47(3), E85-E93.

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For research use only. Not intended for any clinical use.

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