Mouse Anti-AKTIP Recombinant Antibody (V2-160821) (CBMAB-F0749-CQ)

Name: Mouse Anti-AKTIP Recombinant Antibody (V2-160821)
SKU: CBMAB-F0749-CQ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-AKTIP Recombinant Antibody (CBXF-0099) in IP

IP shows binding between FTS and E6/E7 in CaSki and SiHa cells, expression and co-localization of FTS (green color) and HPV16 E6 (brown color) in cervical cancer tissues. ...View More

DS, P., Chaturvedi, P. K., Krishnamoorthy, D., Seo, Y. S., Thippana, M., & Park, W. Y. (2022). Fused toes homolog, a potential molecular regulator of human papillomavirus type 16 E6 and E7 oncoproteins in cervical cancer. Plos one, 17(4), e0266532.

Mouse Anti-AKTIP Recombinant Antibody (CBXF-0099) in IF

Co-localization of FTS and E6/E7 in FTS silenced cells.
Expression of FTS and E6 in CaSki cells (A), FTS and E7 in CaSki cells (B), FTS and E6 in SiHa cells (C), and FTS and E7 in SiHa cells (D). FTS was stained with Alexa Fluor 488 (green), while E6 or E7 was stained with Alexa Fluor 594 (red). The nuclei were counter-stained with DAPI. The Pearson's coefficient for FTS co-localization with E6/E7 revealed that the co-localization ranged between 0.834-0.938. ...View More

Mouse Anti-AKTIP Recombinant Antibody (CBXF-0099) in WB

Cells were transfected with siRNA targeting the 3' UTR of AKTIP. Eight h later, lentivirus for AKTIP overexpression (OX) was added to the culture for another 72 h prior to protein harvest for western blotting. ERK2 was loading control. ...View More

Ng, A. S., Zhang, S., Mak, V. C., Zhou, Y., Yuen, Y., Sharma, R., ... & Cheung, L. W. (2022). AKTIP loss is enriched in ERα-positive breast cancer for tumorigenesis and confers endocrine resistance. Cell reports, 41(11).

Mouse Anti-AKTIP Recombinant Antibody (CBXF-0099) in IHC

uman breast tumor tissue samples were subjected to immunohistochemical staining using anti-AKTIP or anti-ERα antibody. Top, representative immunohistochemical images. The boxes depict magnified areas. Scale bar, 200 μm. Bottom, heatmap illustrating the correlation between ERα and AKTIP staining intensities. p value of Pearson correlation analysis is shown. ...View More

Mouse Anti-AKTIP Recombinant Antibody (CBXF-0099) in IF

Immunofluorescence analysis shows the colocalization of FTS and Hes1 after 24 h radiation. FTS and Hes1 (20 X) were detected using Alexa Fluor 488 (green) and Alexa Fluor 594 (red), respectively. Yellow color (indicated by arrow) in the merged image represents the co-localization. Nucleus was stained by DAPI (Blue). ...View More

Prabakaran, D. S., Muthusami, S., Sivaraman, T., Yu, J. R., & Park, W. Y. (2019). Silencing of FTS increases radiosensitivity by blocking radiation-induced Notch1 activation and spheroid formation in cervical cancer cells. International journal of biological macromolecules, 126, 1318-1325.

Mouse Anti-AKTIP Recombinant Antibody (CBXF-0099) in WB

Effect of FTS-silencing on cell survival after treatment with
cisplatin. FTS-silencing was confirmed with western blot analysis. ...View More

Subramanian, P. D., An, Z., Yu, J. R., & Park, W. Y. (2016). Silencing of fused toes homolog enhances cisplatin sensitivity in cervical cancer cells by inhibiting epidermal growth factor receptor-mediated repair of DNA damage. Cancer chemotherapy and pharmacology, 78, 753-762.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-160821

Application

WB, IHC-P, ELISA

Immunogen

Recombinant FTS protein of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

AKT Interacting Protein

Introduction

The mouse homolog of this gene produces fused toes and thymic hyperplasia in heterozygous mutant animals while homozygous mutants die in early development. This gene may play a role in apoptosis as these morphological abnormalities are caused by altered patterns of programmed cell death. The protein encoded by this gene is similar to the ubiquitin ligase domain of other ubiquitin-conjugating enzymes but lacks the conserved cysteine residue that enables those enzymes to conjugate ubiquitin to the target protein. This protein interacts directly with serine/threonine kinase protein kinase B (PKB)/Akt and modulates PKB activity by enhancing the phosphorylation of PKB's regulatory sites. Alternative splicing results in two transcript variants encoding the same protein.

Entrez Gene ID

64400

UniProt ID

Q9H8T0

Alternative Names

AKT Interacting Protein; FT1; FTS; Fused Toes (Mouse) Homolog; Fused Toes Homolog (Mouse); Fused Toes Protein Homolog; AKT-Interacting Protein; Fused Toes Homolog;

Function

Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). Regulates apoptosis by enhancing phosphorylation and activation of AKT1. Increases release of TNFSF6 via the AKT1/GSK3B/NFATC1 signaling cascade.

Biological Process

Apoptotic process Source: UniProtKB-KW
Early endosome to late endosome transport Source: UniProtKB
Endosome organization Source: UniProtKB
Endosome to lysosome transport Source: UniProtKB
Lysosome organization Source: UniProtKB
Positive regulation of protein binding Source: MGI
Positive regulation of protein phosphorylation Source: MGI
Protein polyubiquitination Source: GO_Central
Protein transport

Cellular Location

Cell membrane; Cytoplasm

Khalil, M. I., Madere, C., Ghosh, I., Adam, R. M., & De Benedetti, A. (2021). Interaction of TLK1 and AKTIP as a Potential Regulator of AKT Activation in Castration-Resistant Prostate Cancer Progression. Pathophysiology, 28(3), 339-354.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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