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Mouse Anti-ALAS1 Recombinant Antibody (V2-611118) (CBMAB-A0271-LY)

The product is antibody recognizes ALAS1. The antibody 2D5 immunoassay techniques such as: WB, ELISA.
See all ALAS1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-611118
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
ALAS1 (NP_000679, 1 a.a. ~ 98 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
5'-Aminolevulinate Synthase 1
Introduction
Delta-aminolevulinate synthase (ALAS; EC 2.3.1.37) catalyzes the condensation of glycine with succinyl-CoA to form delta-aminolevulinic acid. This nuclear-encoded mitochondrial enzyme is the first and rate-limiting enzyme in the mammalian heme biosynthetic pathway. There are 2 tissue-specific isozymes: a housekeeping enzyme encoded by the ALAS1 gene and an erythroid tissue-specific enzyme encoded by ALAS2 (MIM 301300).[supplied by OMIM
Entrez Gene ID
211
UniProt ID
P13196
Alternative Names
ALAS; ALAS3; ALASH; MIG4
Biological Process
Erythrocyte development Source: GO_Central
Heme biosynthetic process Source: GO_Central
Hemoglobin biosynthetic process Source: GO_Central
Mitochondrion organization Source: Reactome
Protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Regulation of metabolic process Source: Reactome
Response to hypoxia Source: GO_Central
Cellular Location
Mitochondrion matrix

Lahiji, A. P., Anderson, K. E., Chan, A., Simon, A., Desnick, R. J., & Ramanujam, V. S. (2020). 5-Aminolevulinate dehydratase porphyria: Update on hepatic 5-aminolevulinic acid synthase induction and long-term response to hemin. Molecular Genetics and Metabolism, 131(4), 418-423.

Stojanovski, B. M., Hunter, G. A., Na, I., Uversky, V. N., Jiang, R. H., & Ferreira, G. C. (2019). 5-Aminolevulinate synthase catalysis: the catcher in heme biosynthesis. Molecular genetics and metabolism, 128(3), 178-189.

Badawy, A. A. B. (2019). Hypothesis: Metabolic targeting of 5-aminolevulinate synthase by tryptophan and inhibitors of heme utilisation by tryptophan 2, 3-dioxygenase as potential therapies of acute hepatic porphyrias. Medical hypotheses, 131, 109314.

Na, I., Catena, D., Kong, M. J., Ferreira, G. C., & Uversky, V. N. (2018). Anti-correlation between the dynamics of the active site loop and C-terminal tail in relation to the homodimer asymmetry of the mouse erythroid 5-aminolevulinate synthase. International journal of molecular sciences, 19(7), 1899.

Ikushiro, H., Nagami, A., Takai, T., Sawai, T., Shimeno, Y., Hori, H., ... & Yano, T. (2018). Heme-dependent inactivation of 5-aminolevulinate synthase from Caulobacter crescentus. Scientific reports, 8(1), 1-13.

Na, I., DeForte, S., Stojanovski, B. M., Ferreira, G. C., & Uversky, V. N. (2018). Molecular dynamics analysis of the structural and dynamic properties of the functionally enhanced hepta-variant of mouse 5-aminolevulinate synthase. Journal of Biomolecular Structure and Dynamics, 36(1), 152-165.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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