Sign in or Register   Sign in or Register
  |  

Mouse Anti-ALAS1 Recombinant Antibody (V2-180595) (CBMAB-A2131-YC)

Provided herein is a Mouse monoclonal antibody against Human 5'-Aminolevulinate Synthase 1. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all ALAS1 antibodies
Published Data
Fig.1 Shown are representative immunoblots blots for cyt c, COXI, ALASI, and HKII.
...View More

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-180595
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
ALAS1 (NP_000679, 1 a.a. ~ 98 a.a) partial recombinant protein with GST tag.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
5'-Aminolevulinate Synthase 1
Introduction
ALAS1 is the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid
Entrez Gene ID
211
UniProt ID
P13196
Alternative Names
5'-Aminolevulinate Synthase 1; 5-Aminolevulinate Synthase 1; Aminolevulinate, Delta-, Synthase 1; Delta-Aminolevulinate Synthase 1; 5-Aminolevulinic Acid Synthase 1; Delta-ALA Synthase 1; EC 2.3.1.37; ALAS-H;
Biological Process
Erythrocyte development Source: GO_Central
Heme biosynthetic process Source: GO_Central
Hemoglobin biosynthetic process Source: GO_Central
Mitochondrion organization Source: Reactome
Protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Regulation of metabolic process Source: Reactome
Response to hypoxia Source: GO_Central
Cellular Location
Mitochondrion matrix

Lahiji, A. P., Anderson, K. E., Chan, A., Simon, A., Desnick, R. J., & Ramanujam, V. S. (2020). 5-Aminolevulinate dehydratase porphyria: Update on hepatic 5-aminolevulinic acid synthase induction and long-term response to hemin. Molecular Genetics and Metabolism, 131(4), 418-423.

Stojanovski, B. M., Hunter, G. A., Na, I., Uversky, V. N., Jiang, R. H., & Ferreira, G. C. (2019). 5-Aminolevulinate synthase catalysis: the catcher in heme biosynthesis. Molecular genetics and metabolism, 128(3), 178-189.

Badawy, A. A. B. (2019). Hypothesis: Metabolic targeting of 5-aminolevulinate synthase by tryptophan and inhibitors of heme utilisation by tryptophan 2, 3-dioxygenase as potential therapies of acute hepatic porphyrias. Medical hypotheses, 131, 109314.

Na, I., Catena, D., Kong, M. J., Ferreira, G. C., & Uversky, V. N. (2018). Anti-correlation between the dynamics of the active site loop and C-terminal tail in relation to the homodimer asymmetry of the mouse erythroid 5-aminolevulinate synthase. International journal of molecular sciences, 19(7), 1899.

Ikushiro, H., Nagami, A., Takai, T., Sawai, T., Shimeno, Y., Hori, H., ... & Yano, T. (2018). Heme-dependent inactivation of 5-aminolevulinate synthase from Caulobacter crescentus. Scientific reports, 8(1), 1-13.

Na, I., DeForte, S., Stojanovski, B. M., Ferreira, G. C., & Uversky, V. N. (2018). Molecular dynamics analysis of the structural and dynamic properties of the functionally enhanced hepta-variant of mouse 5-aminolevulinate synthase. Journal of Biomolecular Structure and Dynamics, 36(1), 152-165.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-ALAS1 Recombinant Antibody (V2-180595)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare