Mouse Anti-GRK3 Recombinant Antibody (G0803) (V2LY-0226-LY1328)

Name: Mouse Anti-GRK3 Recombinant Antibody (G0803)
SKU: V2LY-0226-LY1328
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-GRK3 Recombinant Antibody (G0803) (V2LY-0226-LY1328) in Immunoblot

To create the HA-tagged GRK protein standards for STARPA, ΔQ-GRK cells (HEK293 cells with knockout of GRK2, 3, 5, and 6), were transiently transfected with HA-tagged GRK2, 3-1, 5, or 6-1. Serial dilutions of lysates were analyzed for their GRK levels using anti-HA antibody. ...View More

Reichel, M., Weitzel, V., Klement, L., Hoffmann, C., & Drube, J. (2022). Suitability of GRK Antibodies for Individual Detection and Quantification of GRK Isoforms in Western Blots. International journal of molecular sciences, 23(3), 1195. https://doi.org/10.3390/ijms23031195

Mouse Anti-GRK3 Recombinant Antibody (G0803) (V2LY-0226-LY1328) in Immunoblot

Close ups of the STARPA standards from the immunoblot presented displaying the cross-reactivity of the anti-GRK2 and anti-GRK6 antibody towards GRK3 and GRK5, respectively. Below, the quantification of three independent blots ± SD. The means of the obtained signals are indicated and were used to determine the respective cross-reactivity coefficient of the antibodies. ...View More

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

G0803

Application

ELISA, IP, IF, WB

Immunogen

Amino acids 646-688 mapping at the C-terminus of human GRK3.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

G protein-coupled receptor kinase 3

Entrez Gene ID

157

UniProt ID

P35626

Function

Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors.

Biological Process

Desensitization of G protein-coupled receptor signaling pathway Source: GO_Central
G protein-coupled receptor signaling pathway Source: GO_Central
Protein phosphorylation Source: GO_Central
Receptor internalization Source: UniProtKB
Regulation of signal transduction Source: GO_Central
Signal transduction Source: ProtInc

Cellular Location

Cytosol; Plasma membrane

PTM

Ubiquitinated.

Rabjohns, E. M., Rampersad, R. R., Ghosh, A., Hurst, K., Eudy, A. M., Brozowski, J. M., ... & Tarrant, T. K. (2023). Aged G Protein-Coupled Receptor Kinase 3 (Grk3)-Deficient Mice Exhibit Enhanced Osteoclastogenesis and Develop Bone Lesions Analogous to Human Paget’s Disease of Bone. Cells, 12(7), 981.

Gazdarica, M., Noda, J., Durydivka, O., Novosadova, V., Mackie, K., Pin, J. P., ... & Blahos, J. (2022). SGIP1 modulates kinetics and interactions of the cannabinoid receptor 1 and G protein‐coupled receptor kinase 3 signalosome. Journal of Neurochemistry, 160(6), 625-642.

Brozowski, J. M., Timoshchenko, R. G., Serafin, D. S., Allyn, B., Koontz, J., Rabjohns, E. M., ... & Tarrant, T. K. (2022). G protein-coupled receptor kinase 3 modulates mesenchymal stem cell proliferation and differentiation through sphingosine-1-phosphate receptor regulation. Stem cell research & therapy, 13(1), 37.

Ihara, S., & Touhara, K. (2018). G Protein-Coupled Receptor Kinase 3 (GRK3) in Olfaction. Olfactory Receptors: Methods and Protocols, 33-41.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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