Mouse Egfl7 ELISA Kit (V2LY-0626-LY5824)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
1.65 ng/mL
Detection Range
3-900 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
29.8 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
EGF Like Domain Multiple 7
Research Area
Regulates vascular tubulogenesis in vivo. Inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cell adhesion to the extracellular matrix and angiogenesis.
Biological Process
Anatomical structure development Source: GO_Central
Angiogenesis Source: UniProtKB-KW
Blood vessel development Source: UniProtKB
Cell adhesion Source: UniProtKB-KW
Negative regulation of Notch signaling pathway Source: MGI
Positive regulation of endothelial cell proliferation Source: MGI
Vasculogenesis Source: UniProtKB
Cellular Location
Extracellular space

Ortega, M. A., Asunsolo, A., Fraile‑Martínez, O., Sainz, F., Saez, M. A., Bravo, C., ... & García‑Honduvilla, N. (2021). An increase in elastogenic components in the placental villi of women with chronic venous disease during pregnancy is associated with decreased EGFL7 expression level. Molecular medicine reports, 24(2), 1-9.

Heissig, B., Salama, Y., Takahashi, S., Okumura, K., & Hattori, K. (2021). The multifaceted roles of EGFL7 in cancer and drug resistance. Cancers, 13(5), 1014.

Massimiani, M., Salvi, S., Tiralongo, G. M., Moresi, S., Stuhlmann, H., Valensise, H., ... & Campagnolo, L. (2021). Circulating EGFL7 distinguishes between IUGR and PE: An observational case–control study. Scientific Reports, 11(1), 1-8.

Bill, M., Pathmanathan, A., Karunasiri, M., Shen, C., Burke, M. H., Ranganathan, P., ... & Dorrance, A. M. (2020). EGFL7 Antagonizes NOTCH Signaling and Represents a Novel Therapeutic Target in Acute Myeloid LeukemiaTargeting EGFL7 in AML. Clinical Cancer Research, 26(3), 669-678.

Liu, Q., He, H., Yuan, Y., Zeng, H., Wang, Z., & Luo, W. (2020). Novel expression of EGFL7 in osteosarcoma and sensitivity to cisplatin. Frontiers in Oncology, 10, 74.

Usuba, R., Pauty, J., Soncin, F., & Matsunaga, Y. T. (2019). EGFL7 regulates sprouting angiogenesis and endothelial integrity in a human blood vessel model. Biomaterials, 197, 305-316.

Richter, A., Alexdottir, M. S., Magnus, S. H., Richter, T. R., Morikawa, M., Zwijsen, A., & Valdimarsdottir, G. (2019). EGFL7 mediates BMP9-induced sprouting angiogenesis of endothelial cells derived from human embryonic stem cells. Stem cell reports, 12(6), 1250-1259.

Larochelle, C., Uphaus, T., Broux, B., Gowing, E., Paterka, M., Michel, L., ... & Zipp, F. (2018). EGFL7 reduces CNS inflammation in mouse. Nature communications, 9(1), 1-12.

Dudvarski Stanković, N., Bicker, F., Keller, S., Jones, D. T., Harter, P. N., Kienzle, A., ... & Schmidt, M. H. (2018). EGFL7 enhances surface expression of integrin α5β1 to promote angiogenesis in malignant brain tumors. EMBO molecular medicine, 10(9), e8420.

Hong, G., Kuek, V., Shi, J., Zhou, L., Han, X., He, W., ... & Xu, J. (2018). EGFL7: Master regulator of cancer pathogenesis, angiogenesis and an emerging mediator of bone homeostasis. Journal of cellular physiology, 233(11), 8526-8537.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

EGFL7 Matched Antibody Pair (368) (CAT#: APMAB-368LY)

EGFL7 Matched Antibody Pair (369) (CAT#: APMAB-369LY)

EGFL7 Matched Antibody Pair (370) (CAT#: APMAB-370LY)

Human Recombinant EGFL7 protein, His Tag (CAT#: V2LY-0526-LY3697)

Mouse Recombinant EGFL7 protein, His Tag (CAT#: V2LY-0526-LY8225)

Antibody Pairs

EGFL7 Matched Antibody Pair (368) (CAT#: APMAB-368LY)

EGFL7 Matched Antibody Pair (369) (CAT#: APMAB-369LY)

EGFL7 Matched Antibody Pair (370) (CAT#: APMAB-370LY)

Related Products

Mouse Anti-EGFL7 Recombinant Antibody (CBFYE-0043) (CAT#: CBMAB-E0097-FY)

EGFL7 Matched Antibody Pair (368) (CAT#: APMAB-368LY)

EGFL7 Matched Antibody Pair (369) (CAT#: APMAB-369LY)

Mouse Recombinant EGFL7 protein, His Tag (CAT#: V2LY-0526-LY8225)

Mouse Anti-EGFL7 Recombinant Antibody (2H6) (CAT#: V2LY-0425-LY1547)

Mouse Anti-EGFL7 Recombinant Antibody (3G1) (CAT#: V2LY-0425-LY1548)

Rat Anti-EGFL7 Recombinant Antibody (CBYY-1024) (CAT#: CBMAB-1028-YY)

Human Recombinant EGFL7 protein, His Tag (CAT#: V2LY-0526-LY3697)

Human Anti-EGFL7 Recombinant Antibody (CBFYE-0540) (CAT#: V2LY-0425-LY1542)

Mouse Anti-EGFL7 Recombinant Antibody (CBFYE-0542) (CAT#: V2LY-0425-LY1544)

Isotype control

For research use only. Not intended for any clinical use.

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