Mouse Il12a ELISA Kit (V2LY-0626-LY5852)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.0073 ng/mL
Detection Range
0.015-3 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
24.2 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Interleukin 12A
Function
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Biological Process
Cell migration Source: UniProtKB
Cellular response to virus Source: UniProtKB
Defense response to Gram-positive bacterium Source: UniProtKB
Extrinsic apoptotic signaling pathway Source: BHF-UCL
Immune response Source: UniProtKB
Negative regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis Source: ARUK-UCL
Negative regulation of interleukin-17 production Source: BHF-UCL
Negative regulation of protein secretion Source: ARUK-UCL
Negative regulation of smooth muscle cell proliferation Source: BHF-UCL
Negative regulation of vascular endothelial growth factor signaling pathway Source: ARUK-UCL
Positive regulation of cell adhesion Source: UniProtKB
Positive regulation of dendritic cell chemotaxis Source: UniProtKB
Positive regulation of interferon-gamma production Source: ComplexPortal
Positive regulation of lymphocyte proliferation Source: UniProtKB
Positive regulation of mononuclear cell proliferation Source: AgBase
Positive regulation of natural killer cell activation Source: UniProtKB
Positive regulation of natural killer cell mediated cytotoxicity Source: UniProtKB
Positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target Source: UniProtKB
Positive regulation of natural killer cell proliferation Source: ComplexPortal
Positive regulation of NK T cell activation Source: BHF-UCL
Positive regulation of NK T cell proliferation Source: ComplexPortal
Positive regulation of receptor signaling pathway via JAK-STAT Source: ComplexPortal
Positive regulation of smooth muscle cell apoptotic process Source: BHF-UCL
Positive regulation of T cell mediated cytotoxicity Source: UniProtKB
Positive regulation of T cell proliferation Source: ComplexPortal
Positive regulation of tyrosine phosphorylation of STAT protein Source: UniProtKB
Response to lipopolysaccharide Source: UniProtKB
Response to UV-B Source: UniProtKB
Response to virus Source: UniProtKB
Cellular Location
Secreted

Mohsen, R. T., Al-Azzawi, R. H., & Ad’hiah, A. H. (2022). A single-nucleotide polymorphism of IL12A gene (rs582537 A/C/G) and susceptibility to chronic hepatitis B virus infection among Iraqi patients. Egyptian Journal of Medical Human Genetics, 23(1), 1-3.

Wang, Z., Liu, M., Ye, D., Ye, J., Wang, M., Liu, J., ... & Wan, J. (2021). Il12a deletion aggravates sepsis-induced cardiac dysfunction by regulating macrophage polarization. Frontiers in Pharmacology, 12, 632912.

Nasser Dhabaan, A. A., & Mahdi, M. A. H. (2020). Association between IL12A Gene of G/A genotype Polymorphism and Pulmonary Tuberculosis Risk in Baghdad Population. Indian Journal of Forensic Medicine & Toxicology, 14(3).

Yu, C. R., Choi, J. K., Uche, A. N., & Egwuagu, C. E. (2018). Production of IL-35 by Bregs is mediated through binding of BATF-IRF-4-IRF-8 complex to il12a and ebi3 promoter elements. Journal of Leukocyte Biology, 104(6), 1147-1157.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Cynomolgus Recombinant IL12A protein (CAT#: V2LY-0526-LY1546)

Human Recombinant IL12A protein, hFc Tag (CAT#: V2LY-0526-LY4723)

Human Recombinant IL12A protein, His Tag (CAT#: V2LY-0526-LY4724)

Human Recombinant IL12A protein, His Tag-1 (CAT#: V2LY-0526-LY4727)

Human Recombinant IL12A protein, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY4728)

Human Recombinant IL12A protein, His Tag-2 (CAT#: V2LY-0526-LY4730)

Human Recombinant IL12A protein (CAT#: V2LY-0526-LY4731)

Human Recombinant IL12A protein-1 (CAT#: V2LY-0526-LY4732)

Mouse Recombinant IL12A protein, hFc Tag (CAT#: V2LY-0526-LY8468)

Mouse Recombinant IL12A protein, His & hFc Tag (CAT#: V2LY-0526-LY8469)

Related Products

Mouse Anti-IL12A Recombinant Antibody (CBYC-P086) (CAT#: CBMAB-P0394-YC)

Mouse Anti-IL12A Recombinant Antibody (CBYY-I1294) (CAT#: CBMAB-I2463-YY)

Mouse Recombinant IL12A protein, His & hFc Tag-1 (CAT#: V2LY-0526-LY8470)

Human Recombinant IL12A protein (CAT#: V2LY-0526-LY4731)

Rat Anti-IL12A Recombinant Antibody (CBYY-I1399) (CAT#: CBMAB-I2569-YY)

Mouse Recombinant IL12A protein, hFc Tag (CAT#: V2LY-0526-LY8468)

Rabbit Anti-IL12A Recombinant Antibody (CBYY-I1744) (CAT#: CBMAB-I2914-YY)

Mouse Anti-IL12A Recombinant Antibody (CBYY-I0129) (CAT#: CBMAB-I0370-YY)

Rat Anti-IL12A Recombinant Antibody (CBYY-I1384) (CAT#: CBMAB-I2554-YY)

Mouse Anti-IL12A Recombinant Antibody (CBYY-I1945) (CAT#: CBMAB-I3115-YY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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