Mouse Il24 ELISA Kit (V2LY-0626-LY5864)

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Tested Data
Datasheet
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Basic Information

Sensitivity
0.0093 ng/mL
Detection Range
0.02-4.5 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
20.8 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Interleukin 24
Function
Has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation.
Biological Process
Apoptotic processManual Assertion Based On ExperimentTAS:ProtInc
Cellular response to interleukin-4IEA:Ensembl
Cellular response to lipopolysaccharideIEA:Ensembl
Negative regulation of cell migrationIEA:Ensembl
Negative regulation of cell population proliferationIEA:Ensembl
Positive regulation of cell population proliferationIEA:Ensembl
Positive regulation of tyrosine phosphorylation of STAT proteinIEA:Ensembl
Serine phosphorylation of STAT proteinIEA:Ensembl
Wound healingIEA:Ensembl
Cellular Location
Secreted
PTM
Ubiquitination at Lys-122 promotes proteasomal degradation.

Sie, C., Kant, R., Peter, C., Muschaweckh, A., Pfaller, M., Nirschl, L., ... & Korn, T. (2022). IL-24 intrinsically regulates Th17 cell pathogenicity in mice. Journal of Experimental Medicine, 219(8), e20212443.

Feng, K. N., Meng, P., Zou, X. L., Zhang, M., Li, H. K., Yang, H. L., ... & Zhang, T. T. (2022). IL-37 protects against airway remodeling by reversing bronchial epithelial–mesenchymal transition via IL-24 signaling pathway in chronic asthma. Respiratory Research, 23(1), 1-17.

Davidson, S., Yu, C. H., Steiner, A., Ebstein, F., Baker, P. J., Jarur-Chamy, V., ... & Masters, S. L. (2022). Protein kinase R is an innate immune sensor of proteotoxic stress via accumulation of cytoplasmic IL-24. Science Immunology, 7(68), eabi6763.

Chong, W. P., Mattapallil, M. J., Raychaudhuri, K., Bing, S. J., Wu, S., Zhong, Y., ... & Caspi, R. R. (2020). The cytokine IL-17A limits Th17 pathogenicity via a negative feedback loop driven by autocrine induction of IL-24. Immunity, 53(2), 384-397.

Emdad, L., Bhoopathi, P., Talukdar, S., Pradhan, A. K., Sarkar, D., Wang, X. Y., ... & Fisher, P. B. (2020, November). Recent insights into apoptosis and toxic autophagy: The roles of MDA-7/IL-24, a multidimensional anti-cancer therapeutic. In Seminars in cancer biology (Vol. 66, pp. 140-154). Academic Press.

Mitamura, Y., Nunomura, S., Furue, M., & Izuhara, K. (2020). IL-24: A new player in the pathogenesis of pro-inflammatory and allergic skin diseases. Allergology International, 69(3), 405-411.

Schmetzer, O., Lakin, E., Topal, F. A., Preusse, P., Freier, D., Church, M. K., & Maurer, M. (2018). IL-24 is a common and specific autoantigen of IgE in patients with chronic spontaneous urticaria. Journal of Allergy and Clinical Immunology, 142(3), 876-882.

Mitamura, Y., Nunomura, S., Nanri, Y., Ogawa, M., Yoshihara, T., Masuoka, M., ... & Izuhara, K. (2018). The IL‐13/periostin/IL‐24 pathway causes epidermal barrier dysfunction in allergic skin inflammation. Allergy, 73(9), 1881-1891.

Menezes, M. E., Bhoopathi, P., Pradhan, A. K., Emdad, L., Das, S. K., Guo, C., ... & Fisher, P. B. (2018). Role of MDA-7/IL-24 a multifunction protein in human diseases. Advances in cancer research, 138, 143-182.

Pradhan, A. K., Bhoopathi, P., Talukdar, S., Shen, X. N., Emdad, L., Das, S. K., ... & Fisher, P. B. (2018). Recombinant MDA-7/IL24 suppresses prostate cancer bone metastasis through downregulation of the Akt/Mcl-1 pathway. Molecular cancer therapeutics, 17(9), 1951-1960.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant IL24 protein, His Tag (CAT#: V2LY-0526-LY4861)

Human Recombinant IL24 protein, His Tag-1 (CAT#: V2LY-0526-LY4862)

Human Recombinant IL24 protein, Fc Tag (CAT#: V2LY-0526-LY4863)

Human Recombinant IL24 protein, Fc Tag-1 (CAT#: V2LY-0526-LY4864)

Rat Il24 ELISA Kit (CAT#: V2LY-0626-LY1653)

Human IL24 ELISA Kit (CAT#: V2LY-0626-LY2448)

Related Products

Human Recombinant IL24 protein, Fc Tag (CAT#: V2LY-0526-LY4863)

Human IL24 ELISA Kit (CAT#: V2LY-0626-LY2448)

Mouse Anti-IL24 Recombinant Antibody (B-C60) (CAT#: CBMAB-I1981-YY)

Rat Il24 ELISA Kit (CAT#: V2LY-0626-LY1653)

Human Recombinant IL24 protein, His Tag (CAT#: V2LY-0526-LY4861)

Mouse Anti-IL24 Recombinant Antibody (CBYY-I0191) (CAT#: CBMAB-I0517-YY)

Mouse Anti-IL24 Recombinant Antibody (CBYY-I1196) (CAT#: CBMAB-I2365-YY)

Mouse Anti-IL24 Recombinant Antibody (CBYY-I0889) (CAT#: CBMAB-I2057-YY)

Mouse Anti-IL24 Recombinant Antibody (A540) (CAT#: CBMAB-AP3953LY)

Human Recombinant IL24 protein, Fc Tag-1 (CAT#: V2LY-0526-LY4864)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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