Mouse Il9 ELISA Kit (V2LY-0626-LY314)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.0052 ng/mL
Detection Range
0.01-2 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
16.1 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Interleukin 9
Function
Supports IL-2 independent and IL-4 independent growth of helper T-cells.
Biological Process
B cell differentiationIEA:Ensembl
B cell proliferationIEA:Ensembl
Immunoglobulin mediated immune responseIEA:Ensembl
Inflammatory responseManual Assertion Based On ExperimentTAS:ProtInc
Negative regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathwayIEA:Ensembl
Positive regulation of cell growthIEA:Ensembl
Positive regulation of cell population proliferationManual Assertion Based On ExperimentTAS:ProtInc
Positive regulation of interleukin-5 productionISS:UniProtKB
Regulation of peptidyl-tyrosine phosphorylationIEA:Ensembl
Regulation of receptor signaling pathway via JAK-STATIEA:Ensembl
Cellular Location
Secreted

Kharwadkar, R., Ulrich, B. J., Chu, M., Koh, B., Hufford, M. M., Fu, Y., ... & Kaplan, M. H. (2023). ERG functionally overlaps with other Ets proteins in promoting TH9 cell expression of Il9 during allergic lung inflammation. The Journal of Immunology, 210(5), 537-546.

Sohn, J., Li, L., Zhang, L., Settem, R. P., Honma, K., Sharma, A., ... & Kirkwood, K. L. (2022). Porphyromonas gingivalis indirectly elicits intestinal inflammation by altering the gut microbiota and disrupting epithelial barrier function through IL9‐producing CD4+ T cells. Molecular Oral Microbiology, 37(2), 42-52.

Zha, L., Dong, J., Chen, Q., Liao, Y., Zhang, H., Xie, T., ... & Nie, S. (2022). Genetic association analysis between IL9 and coronary artery disease in a Chinese Han population. Cytokine, 150, 155761.

Chen, Q., Wang, W., Khanal, S., Han, J., Zhang, M., Chen, Y., ... & Wang, B. (2021). Transcriptome analysis reveals genes potentially related to high fiber strength in a Gossypium hirsutum line IL9 with Gossypium mustelinum introgression. Genome, 64(11), 985-995.

Sun, Y., Liu, S., Hu, R., Zhou, Q., & Li, X. (2020). Decreased placental IL9 and IL9R in preeclampsia impair trophoblast cell proliferation, invasion, and angiogenesis. Hypertension in Pregnancy, 39(3), 228-235.

Fu, Y., Wang, J., Panangipalli, G., Ulrich, B. J., Koh, B., Xu, C., ... & Kaplan, M. H. (2020). STAT5 promotes accessibility and is required for BATF-mediated plasticity at the Il9 locus. Nature communications, 11(1), 4882.

Lee, W. H., Jang, S. W., Kim, H. S., Kim, S. H., Heo, J. I., Kim, G. E., & Lee, G. R. (2019). BATF3 is sufficient for the induction of Il9 expression and can compensate for BATF during Th9 cell differentiation. Experimental & molecular medicine, 51(11), 1-12.

Abdul Qayum, A., Koh, B., Martin, R. K., Kenworthy, B. T., Kharwadkar, R., Fu, Y., ... & Kaplan, M. H. (2019). The Il9 CNS-25 regulatory element controls mast cell and basophil IL-9 production. The Journal of Immunology, 203(5), 1111-1121.

Li, L., Huang, S., Wang, H., Chao, K., Ding, L., Feng, R., ... & Zhang, S. (2018). Cytokine IL9 triggers the pathogenesis of inflammatory bowel disease through the miR21-CLDN8 pathway. Inflammatory Bowel Diseases, 24(10), 2211-2223.

Koh, B., Abdul Qayum, A., Srivastava, R., Fu, Y., Ulrich, B. J., Janga, S. C., & Kaplan, M. H. (2018). A conserved enhancer regulates Il9 expression in multiple lineages. Nature communications, 9(1), 4803.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant IL9 protein, His Tag (CAT#: V2LY-0526-LY4969)

Mouse Recombinant IL9 protein, His Tag (CAT#: V2LY-0526-LY8555)

Rat Recombinant IL9 protein, His Tag (CAT#: V2LY-0526-LY9487)

Human IL9 ELISA Kit (CAT#: V2LY-0626-LY334)

Related Products

Recombinant Hamster Anti-IL9 Recombinant Antibody (D9302C12) (CAT#: CBMAB-XB0612-YC)

Rat Anti-IL9 Recombinant Antibody (CBYY-I0450) (CAT#: CBMAB-I1050-YY)

Recombinant Mouse Anti-IL9 Recombinant Antibody (9D1) (CAT#: CBMAB-XB0610-YC)

Rat Recombinant IL9 protein, His Tag (CAT#: V2LY-0526-LY9487)

Mouse Anti-IL9 Recombinant Antibody (A614) (CAT#: CBMAB-AP10871LY)

Mouse Anti-IL9 Recombinant Antibody (CBYY-I0327) (CAT#: CBMAB-I0863-YY)

Recombinant Rat Anti-IL9 Recombinant Antibody (RM9A4) (CAT#: CBMAB-XB0613-YC)

Mouse Anti-IL9 Recombinant Antibody (1C9) (CAT#: CBMAB-A4449-LY)

Mouse Anti-IL9 Recombinant Antibody (CF243) (CAT#: CBMAB-FT281LY)

Mouse Anti-IL9 Recombinant Antibody (9C1) (CAT#: CBMAB-I1830-YY)

Isotype control

For research use only. Not intended for any clinical use.

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