Mouse Recombinant IL9 protein, His Tag (V2LY-0526-LY8555)

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Basic Information

Expressed Host
Baculovirus-Insect Cells
Protein Species
Mouse
Tag
His Tag
Protein Construction
This product is Mouse Recombinant IL9 protein, His Tag consist of Amino Acid: 1-144 and predicts a molecular mass of 15.6 kDa.
Molecule Mass
15.6 kDa
Sequence
Amino Acid: 1-144
Species
Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
>95% as determined by SDS-PAGE.
Endotoxin
Please contact us for more information.
Format
Lyophilized
Reconstitution
Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.
Buffer
Lyophilized from sterile Tirs, NaCl, Glycerol
Preservative
None
Storage
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
More Infomation

Target

Full Name
Interleukin 9
Function
Supports IL-2 independent and IL-4 independent growth of helper T-cells.
Biological Process
B cell differentiationIEA:Ensembl
B cell proliferationIEA:Ensembl
Immunoglobulin mediated immune responseIEA:Ensembl
Inflammatory responseManual Assertion Based On ExperimentTAS:ProtInc
Negative regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathwayIEA:Ensembl
Positive regulation of cell growthIEA:Ensembl
Positive regulation of cell population proliferationManual Assertion Based On ExperimentTAS:ProtInc
Positive regulation of interleukin-5 productionISS:UniProtKB
Regulation of peptidyl-tyrosine phosphorylationIEA:Ensembl
Regulation of receptor signaling pathway via JAK-STATIEA:Ensembl
Cellular Location
Secreted

Kharwadkar, R., Ulrich, B. J., Chu, M., Koh, B., Hufford, M. M., Fu, Y., ... & Kaplan, M. H. (2023). ERG functionally overlaps with other Ets proteins in promoting TH9 cell expression of Il9 during allergic lung inflammation. The Journal of Immunology, 210(5), 537-546.

Sohn, J., Li, L., Zhang, L., Settem, R. P., Honma, K., Sharma, A., ... & Kirkwood, K. L. (2022). Porphyromonas gingivalis indirectly elicits intestinal inflammation by altering the gut microbiota and disrupting epithelial barrier function through IL9‐producing CD4+ T cells. Molecular Oral Microbiology, 37(2), 42-52.

Zha, L., Dong, J., Chen, Q., Liao, Y., Zhang, H., Xie, T., ... & Nie, S. (2022). Genetic association analysis between IL9 and coronary artery disease in a Chinese Han population. Cytokine, 150, 155761.

Chen, Q., Wang, W., Khanal, S., Han, J., Zhang, M., Chen, Y., ... & Wang, B. (2021). Transcriptome analysis reveals genes potentially related to high fiber strength in a Gossypium hirsutum line IL9 with Gossypium mustelinum introgression. Genome, 64(11), 985-995.

Sun, Y., Liu, S., Hu, R., Zhou, Q., & Li, X. (2020). Decreased placental IL9 and IL9R in preeclampsia impair trophoblast cell proliferation, invasion, and angiogenesis. Hypertension in Pregnancy, 39(3), 228-235.

Fu, Y., Wang, J., Panangipalli, G., Ulrich, B. J., Koh, B., Xu, C., ... & Kaplan, M. H. (2020). STAT5 promotes accessibility and is required for BATF-mediated plasticity at the Il9 locus. Nature communications, 11(1), 4882.

Lee, W. H., Jang, S. W., Kim, H. S., Kim, S. H., Heo, J. I., Kim, G. E., & Lee, G. R. (2019). BATF3 is sufficient for the induction of Il9 expression and can compensate for BATF during Th9 cell differentiation. Experimental & molecular medicine, 51(11), 1-12.

Abdul Qayum, A., Koh, B., Martin, R. K., Kenworthy, B. T., Kharwadkar, R., Fu, Y., ... & Kaplan, M. H. (2019). The Il9 CNS-25 regulatory element controls mast cell and basophil IL-9 production. The Journal of Immunology, 203(5), 1111-1121.

Li, L., Huang, S., Wang, H., Chao, K., Ding, L., Feng, R., ... & Zhang, S. (2018). Cytokine IL9 triggers the pathogenesis of inflammatory bowel disease through the miR21-CLDN8 pathway. Inflammatory Bowel Diseases, 24(10), 2211-2223.

Koh, B., Abdul Qayum, A., Srivastava, R., Fu, Y., Ulrich, B. J., Janga, S. C., & Kaplan, M. H. (2018). A conserved enhancer regulates Il9 expression in multiple lineages. Nature communications, 9(1), 4803.

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For research use only. Not intended for any clinical use.

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